An improved microbial assay for the detection of chloramphenicol residues in shrimp tissues

被引：39

作者：

Shakila, R. Jeya ^{[1
,2
]}

Saravanakumar, R. ^{[1
,2
]}

Vyla, S. Aunto Princy ^{[1
,2
]}

Jeyasekaran, G. ^{[1
,2
]}

机构：

[1] Tamil Nadu Vet & Anim Sci Univ, Coll Fisheries, Dept Fish Proc Technol, Tuticorin 628008, India

[2] Tamil Nadu Vet & Anim Sci Univ, Res Inst, Tuticorin 628008, India

来源：

INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES | 2007年 / 8卷 / 04期

关键词：

shrimps; chloramphenicol; luminescent bacteria; microbial assay; pad plate technique;

D O I：

10.1016/j.ifset.2007.03.002

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

An improved microbial assay was developed for the detection of chloramphenicol (CAP) residues in shrimp muscle using Photobacterium leiognathi (L-2 strain) isolated from squid, as test organism, using a pad plate technique. CAP spiked in shrimp muscle tissues at concentrations ranging from 1 to 100 mu g/kg was extracted using ethyl acetate and ammonium hydroxide. The assay showed a maximum inhibition zone of 23.0 min for 100 mu g/kg and a minimum inhibition zone of 9.3 mm for 1 mu g/kg. The method had a good recovery of 95.63% with a minimum detection limit of 1 mu g/kg. It can be performed within 18 h following simple extraction. The method therefore proved to be advantageous over chromatographic procedures as it was inexpensive, quite sensitive and can be adopted for rapid screening of CAP in shrimp tissues. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：515 / 518

页数：4

共 16 条

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