Limitations of specific reverse-transcriptase polymerase chain reaction markers in the detection of metastases in the lymph nodes and blood of breast cancer patients

被引:312
作者
Bostick, PJ
Chatterjee, S
Chi, DD
Huynh, KT
Giuliano, AE
Cote, R
Hoon, DSB
机构
[1] St Johns Hlth Ctr, John Wayne Canc Inst, Joyce Eisenberg Keefer Breast Ctr, Dept Mol Oncol, Santa Monica, CA 90404 USA
[2] Univ So Calif, Sch Med, Dept Pathol, Los Angeles, CA 90033 USA
关键词
D O I
10.1200/JCO.1998.16.8.2632
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Purpose: This study was performed to evaluate the potential of specific mRNA markers to detect micrometastases by reverse-transcriptase polymerase chain reaction (RT-PCR) and Southern blot analysis of sentinel lymph nodes (SNs) and blood from patients with breast cancer. Patients and Methods: We assessed the specificity of carcinoembryonic antigen (CEA), cytokeratin-19 (CK-19), CK-20, gastrointestinal tumor-associated antigen-733.2 (GA733.2), and mucin-1 (MUC-1) in the blood of healthy donors (n = 13) and lymph nodes from patients without cancer (n = 3) by RT-PCR assay. The sensitivity of the RT-PCR assay for the target mRNA markers was assessed in breast cancer cell lines (n = 4), primary breast tumors (n = 8), and the frozen sections of SNs (n = 22) from 22 patients with American Joint Committee on Cancer (AJCC) stages I to IIIA breast cancer. Results: CK-20 was the only mRNA marker not detected in lymph nodes or blood from patients without cancer. Both the blood and lymph nodes from patients without cancer expressed CEA, CK-19, GA733.2, and MUG-I mRNA, All four breast cancer cell lined and six of eight primary breast tumors expressed all five mRNA markers. Expression of mRNA by the RT-PCR assay in the frozen-section SNs (n = 12) without metastases by conventional histopathology ranged from 8% (CK-20) to 92% (GA733.2). Detection of RT-PCR cDNA products in frozen-section SNs war increased with Southern blot analysis compared with ethidium bromide gel electrophoresis (EtBr) for all mRNA markers except CK-19. Conclusion: CEA,CK-19, GA733.2, and MUC-1 show no diagnostic value as mRNA markers for the detection of micrometastases by the RT-PCR assay because they are expressed in the blood and lymph nodes of patients without cancer. Further studies are needed to assess the sensitivity of CK-20 to detect micrometastases by the RT-PCR assay in the blood and frozen-section SNs of patients with breast cancer, J Clin Oncol 16:2632-2640. (C) 1998 by American Society of Clinical Oncology.
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页码:2632 / 2640
页数:9
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