Rapid clinical diagnostics assays using injection-molded planar waveguides

被引：9

作者：

Herron, JN ^{[1
]}

Wang, HK ^{[1
]}

Terry, AH ^{[1
]}

Durtschi, JD ^{[1
]}

Tan, L ^{[1
]}

Astill, ME ^{[1
]}

Smith, RS ^{[1
]}

Christensen, DA ^{[1
]}

机构：

[1] Univ Utah, Dept Pharmaceut, Salt Lake City, UT 84112 USA

来源：

SYSTEMS AND TECHNOLOGIES FOR CLINICAL DIAGNOSTICS AND DRUG DISCOVERY, PROCEEDINGS OF | 1998年 / 3259卷

关键词：

biosensors; CK-MB; cTnI; diagnostics; evanescence; fluorescence; hCG; immunoassays; waveguides;

D O I：

10.1117/12.307322

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The goal of our research program is to develop an evanescent wave immunoassay system that can be used in point-of-care and critical care settings. Several key attributes are required to accomplish this goal: (i) the assay system should be at least as sensitive as present day immunoassays; (ii) assay time should be 5 minutes or less; (iii) the assay protocol should be relatively simple; (iv) the sensor should be capable of performing more than one assay on a single specimen; (v) the assay system should be able to accommodate specimens such as serum, plasma and whole blood; and (vi) the sensor should be an inexpensive, disposable cartridge. Our laboratory has developed an injection-molded planar waveguide sensor that meets most, if not all, of these attributes. This sensor has been evaluated in a number of different immunoassays for analytes such as bovine serum albumin, human chorionic gonadotrophin, creatine phosphokinase MB and cardiac troponin I.

引用

页码：54 / 64

页数：11