Immunolocalization of OV-6, a putative progenitor cell marker in human fetal and diseased pediatric liver

被引:96
作者
Crosby, HA [1 ]
Hubscher, SG
Joplin, RE
Kelly, DA
Strain, AJ
机构
[1] Queen Elizabeth Hosp, Liver Unit, Liver Res Labs, Birmingham B15 2TH, W Midlands, England
[2] Univ Birmingham, Dept Biochem, Birmingham B15 2TT, W Midlands, England
[3] Univ Birmingham, Dept Pathol, Birmingham, W Midlands, England
[4] Univ Birmingham, Dept Med, Birmingham, W Midlands, England
[5] Univ Birmingham, Dept Child Hlth, Birmingham, W Midlands, England
[6] Childrens Hosp, Liver Unit, Birmingham B16 8ET, W Midlands, England
关键词
D O I
10.1002/hep.510280412
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
The existence of progenitor (stem) cells in the human liver remains a matter of debate. In rodent models of hepatocarcinogenesis and injury, oval cells proliferate in the periportal regions of the portal tracts and are suggested to derive from a stem cell compartment, because they are capable of differentiating into hepatocytes or biliary epithelial cells. In this study, the rat oval cell marker, OV-6 has been used to investigate the hypothesis that there are stem cells present in fetal and pediatric human liver. The pattern of OV-6 expression was compared with the established adult biliary cell markers human epithelial antigen-125 (HEA-125) and cytokeratin-19 (CK-19). In normal pediatric liver (n = 7), bile ducts and ductules were immunostained with CK-19 and HEA-125, whereas OV-6 staining was consistently negative. In fetal tissue (n = 10), ductal plate cells, primitive bile ducts, and hepatoblasts were stained with CK-19 and HEA-125 although only some of the ductal plate cells and hepatoblasts were OV-6 positive. In biliary atresia (n = 6) and alpha 1, anti-trypsin deficiency (alpha 1,AT) (n = 4), CK-19 and HEA-125 immunostained ductular proliferative cells that tended to form finely anastomosing ductules, whereas OV-6 staining was found more on discrete cells confined to portal tract margins. Additionally, in diseased liver, OV-6 was strongly positive in hepatocyte lobules with greatest intensity in the periseptal regions. This widespread hepatocyte OV-6 positivity suggests that the antibody may identify cells of a less differentiated phenotype (transitional hepatocytes) that have replaced the mature cells. Therefore, it is proposed that in human liver, OV-6 is recognizing cells with a progenitor stem cell-like phenotype with the capacity to differentiate into OV-6 positive ductular cells or lobular hepatocytes.
引用
收藏
页码:980 / 985
页数:6
相关论文
共 34 条
[1]   KERATIN-14 PROTEIN IN CULTURED NONPARENCHYMAL RAT HEPATIC EPITHELIAL-CELLS - CHARACTERIZATION OF KERATIN-14 AND KERATIN-19 AS ANTIGENS FOR THE COMMONLY USED MOUSE MONOCLONAL-ANTIBODY OV-6 [J].
BISGAARD, HC ;
PARMELEE, DC ;
DUNSFORD, HA ;
SECHI, S ;
THORGEIRSSON, SS .
MOLECULAR CARCINOGENESIS, 1993, 7 (01) :60-66
[2]   HEMATOPOIETIC STEM-CELL MARKERS ARE EXPRESSED BY DUCTAL PLATE AND BILE-DUCT CELLS IN DEVELOPING HUMAN LIVER [J].
BLAKOLMER, K ;
JASKIEWICZ, K ;
DUNSFORD, HA ;
ROBSON, SC .
HEPATOLOGY, 1995, 21 (06) :1510-1516
[3]  
BRAUMANN U, HEPATOLOGY, V26, pA378
[4]   Bile ductule formation in fetal, neonatal, and infant livers compared with extrahepatic biliary atresia [J].
Cocjin, J ;
Rosenthal, P ;
Buslon, V ;
Luk, L ;
Barajas, L ;
Geller, SA ;
Ruebner, B ;
French, S .
HEPATOLOGY, 1996, 24 (03) :568-574
[5]  
COLEMAN WB, 1998, LIVER GROWTH REPAIR, P50
[6]  
Crosby HA, 1998, AM J PATHOL, V152, P771
[7]  
Desmet V. J., 1994, P425
[8]  
DEVOS R, 1992, AM J PATHOL, V140, P1441
[9]  
DUNSFORD HA, 1985, AM J PATHOL, V118, P218
[10]  
DUNSFORD HA, 1989, CANCER RES, V49, P4887