Effect of SK&F96365 on extracellular Ca2+-dependent O2- production in neutrophil-like HL-60 cells

被引：22

作者：

Gallois, A ^{[1
]}

Bueb, JL ^{[1
]}

Tschirhart, E ^{[1
]}

机构：

[1] Ctr Rech Publ Sante, L-1150 Luxembourg, Luxembourg

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 1998年 / 361卷 / 2-3期

关键词：

fMLP (N-formyl-L-methionyl-L-leucyl-L-phenylalanine); Ca2+; intracellular; superoxide; neutrophil; respiratory burst;

D O I：

10.1016/S0014-2999(98)00728-6

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Store-operated Ca2+ entry is referred to a capacitative current activated by Ca2+-stores depletion in various non-excitable cells. Neutrophil-like HL-60 cells responded to N-formyl-L-Methionyl-L-leucyl-L-Phenylalanine (fMLP) by an early O-2(-) production preceded by a [Ca2+](i) rise. Cell stimulation in the absence of extracellular Ca2+ resulted in a major reduction of [Ca2+](i) rise and O-2(-) production. A purported inhibitor of store-operated Ca2+ entry, SK&F 96365 (1-(beta-(3-(4-methoxy-phenyl)propoxyl)-4-methoxy-phenetyl)-1H-imidazole hydrochloride), inhibited extracellular Ca2+-dependent [Ca2+](i) rise by 30% but did not alter O-2(-) production. In conclusion, SK&F 96365 did not modify extracellular Ca2+-dependent O-2(-) production, despite a significant but limited reduction in fMLP-activated membrane Ca2+ fluxes which can be ascribed to store-operated Ca2+ entry. Furthermore, Ca2+ influx is necessary for a full induction and maintenance of the biological response. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：293 / 298

页数：6

共 16 条

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