Characterization of Antibodies Induced by Vaccination with Hepatitis C Virus Envelope Glycoproteins

被引：76

作者：

Ray, Ranjit ^{[1
,2
]}

Meyer, Keith ^{[1
,2
]}

Banerjee, Arup ^{[1
,2
]}

Basu, Arnab ^{[1
,2
]}

Coates, Stephen ^{[3
]}

Abrignani, Sergio ^{[3
]}

Houghton, Michael ^{[3
]}

Frey, Sharon E. ^{[1
,2
]}

Belshe, Robert B. ^{[1
,2
]}

机构：

[1] St Louis Univ, Dept Internal Med, St Louis, MO 63103 USA

[2] St Louis Univ, Vaccine & Treatment Evaluat Unit, St Louis, MO 63103 USA

[3] Novartis Vaccines & Diagnost, Emeryville, CA USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 2010年 / 202卷 / 06期

基金：

美国国家卫生研究院;

关键词：

E1; GLYCOPROTEIN; INFECTION; CHIMPANZEES;

D O I：

10.1086/655902

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Hepatitis C virus (HCV) envelope glycoproteins E1 and E2 were used with MF59 adjuvant as a candidate vaccine for a phase 1 safety and immunogenicity trial. Ten of 41 vaccinee serum samples displayed a neutralization titer of >= 1: 20 against vesicular stomatitis virus (VSV)-HCV pseudotype, 15 of 36 serum samples tested had a neutralization titer of >= 1: 400 against human immunodeficiency virus (HIV)-HCV pseudotype, and 10 of 36 serum samples tested had a neutralization titer of >= 1: 20 against cell culture-grown HCV genotype 1a. Neutralizing serum samples had increased affinity levels and displayed >2-fold higher specific activity levels to well-characterized epitopes on E1/E2, especially to the hypervariable region 1 of E2.

引用

页码：862 / 866

页数：5

共 15 条

[1] VACCINATION OF CHIMPANZEES AGAINST INFECTION BY THE HEPATITIS-C VIRUS [J].

CHOO, QL ;

KUO, G ;

RALSTON, R ;

WEINER, A ;

CHIEN, D ;

VANNEST, G ;

HAN, J ;

BERGER, K ;

THUDIUM, K ;

KUO, C ;

KANSOPON, J ;

MCFARLAND, J ;

TABRIZI, A ;

CHING, K ;

MOSS, B ;

CUMMINS, LB ;

HOUGHTON, M ;

MUCHMORE, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (04) :1294-1298

[2] Development and Characterization of Hepatitis C Virus Genotype 1-7 Cell Culture Systems: Role of CD81 and Scavenger Receptor Class B Type I and Effect of Antiviral Drugs [J].

Gottwein, Judith M. ;

Scheel, Troels K. H. ;

Jensen, Tanja B. ;

Lademann, Jacob B. ;

Prentoe, Jannick C. ;

Knudsen, Maria L. ;

Hoegh, Anne M. ;

Bukh, Jens .

HEPATOLOGY, 2009, 49 (02) :364-377

[3] Hepatitis C virus glycoproteins mediate pH-dependent cell entry of pseudotyped retroviral particles [J].

Hsu, M ;

Zhang, J ;

Flint, M ;

Logvinoff, C ;

Cheng-Mayer, C ;

Rice, CM ;

McKeating, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (12) :7271-7276

[4] Generation of infectious hepatitis C virus in immortalized human hepatocytes [J].

Kanda, T ;

Basu, A ;

Steele, R ;

Wakita, T ;

Ryerse, JS ;

Ray, R ;

Ray, RB .

JOURNAL OF VIROLOGY, 2006, 80 (09) :4633-4639

[5] Functional role of hepatitis C virus chimeric glycoproteins in the infectivity of pseudotyped virus [J].

Lagging, LM ;

Meyer, K ;

Owens, RJ ;

Ray, R .

JOURNAL OF VIROLOGY, 1998, 72 (05) :3539-3546

[6] Neutralization of pseudotyped vesicular stomatitis virus expressing hepatitis C virus envelope glycoprotein 1 or 2 by serum from patients [J].

Lagging, LM ;

Meyer, K ;

Westin, J ;

Wejstål, R ;

Norkrans, G ;

Lindh, M ;

Ray, R .

JOURNAL OF INFECTIOUS DISEASES, 2002, 185 (08) :1165-1169

[7] Isolation and characterization of broadly neutralizing human monoclonal antibodies to the E1 glycoprotein of hepatitis C virus [J].

Meunier, Jean-Christophe ;

Russell, Rodney S. ;

Goossens, Vera ;

Priem, Sofie ;

Walter, Hugo ;

Depla, Erik ;

Union, Ann ;

Faulk, Kristina N. ;

Bukh, Jens ;

Emerson, Suzanne U. ;

Purcell, Robert H. .

JOURNAL OF VIROLOGY, 2008, 82 (02) :966-973

[8] Coexpression of hepatitis C virus E1 and E2 chimeric envelope glycoproteins displays separable ligand sensitivity and increases pseudotype infectious titer [J].

Meyer, K ;

Beyene, A ;

Bowlin, TL ;

Basu, A ;

Ray, R .

JOURNAL OF VIROLOGY, 2004, 78 (23) :12838-12847

[9] Neutralizing antibodies in patients with severe acute respiratory syndrome-associated coronavirus infection [J].

Nie, YC ;

Wang, GW ;

Shi, XL ;

Zhang, H ;

Qiu, Y ;

He, ZP ;

Wang, W ;

Lian, GW ;

Yin, XL ;

Du, LY ;

Ren, LL ;

Wang, JW ;

He, X ;

Li, TS ;

Deng, HK ;

Ding, MX .

JOURNAL OF INFECTIOUS DISEASES, 2004, 190 (06) :1119-1126

[10] PEPTIDE IMMUNOGEN MIMICRY OF PUTATIVE E1 GLYCOPROTEIN-SPECIFIC EPITOPES IN HEPATITIS-C VIRUS [J].

RAY, R ;

KHANNA, A ;

LAGGING, LM ;

MEYER, K ;

CHOO, QL ;

RALSTON, R ;

HOUGHTON, M ;

BECHERER, PR .

JOURNAL OF VIROLOGY, 1994, 68 (07) :4420-4426

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