Chromosome analysis and molecular characterization of highly repeated DNAs in the aphid Acyrthosiphon pisum (Aphididae, Hemiptera)

被引：31

作者：

Bizzaro, D ^{[1
]}

Mandrioli, M ^{[1
]}

Zanotti, M ^{[1
]}

Giusti, M ^{[1
]}

Manicardi, GC ^{[1
]}

机构：

[1] Univ Modena & Reggio Emilia, Dipartimento Biol Anim, I-41100 Modena, Italy

来源：

GENETICA | 2000年 / 108卷 / 02期

关键词：

aphid; holocentric chromosomes; NOR; C-banding; 5S rDNA; telomere;

D O I：

10.1023/A:1004128314728

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Despite the interest in aphid biology, information on chromatin organization of their holocentric chromosomes is still limited to few species. In order to fill this gap, we have performed an extensive survey on pea aphid mitotic chromosomes using both classical and molecular cytogenetic techniques. Our results after silver, CMA(3) and DAPI-staining, C-banding and fluorescent in situ hybridization (FISH) using 28S rDNA and 5S rDNA as probes evidenced a tendency of repetitive DNAs to be concentrated on the X chromosomes. FISH experiments with the telomeric probe (TTAGG)(n) revealed bright hybridization signals on each telomere of all Acyrthosiphon pisum chromosomes. No interstitial signals were seen.

引用

页码：197 / 202

页数：6

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