Exponential DNA replication by laminar convection

被引:112
作者
Braun, D [1 ]
Goddard, NL [1 ]
Libchaber, A [1 ]
机构
[1] Rockefeller Univ, Ctr Studies Phys & Biol, New York, NY 10021 USA
关键词
D O I
10.1103/PhysRevLett.91.158103
中图分类号
O4 [物理学];
学科分类号
0702 ;
摘要
It is shown that laminar thermal convection can drive a chain reaction of DNA replication. The convection is triggered by a constant horizontal temperature gradient, moving molecules along stationary paths between hot and cold regions. This implements the temperature cycling for the classical polymerase chain reaction (PCR). The amplification is shown to be exponential and reaches 100 000-fold gains within 25 min. Besides direct applications, the mechanism might have implications for the molecular evolution of life.
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页数:4
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