PI 3-kinase and MAP kinase regulate bradykinin induced prostaglandin E2 release in human pulmonary artery by modulating COX-2 activity

被引：20

作者：

Bradbury, DA ^{[1
]}

Corbett, L ^{[1
]}

Knox, AJ ^{[1
]}

机构：

[1] Univ Nottingham, City Hosp, Div Resp Med, Nottingham NG5 1PB, England

来源：

FEBS LETTERS | 2004年 / 560卷 / 1-3期

关键词：

bradykinin; cyclooxygenase; prostaglandin E-2; phosphoinositide; 3-kinase; mitogen-activated protein kinase;

D O I：

10.1016/S0014-5793(04)00064-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we studied the role of phosphoinositide 3-kinase (PI 3-kinase) and mitogen activated protein (MAP) kinase in regulating bradykinin (BK) induced prostaglandin E-2 (PGE(2)) production in human pulmonary artery smooth muscle cells (HPASMC). BK increased PGE(2) in a three step process involving phospholipase A(2) (PLA(2)), cyclooxygenase (COX) and PGE synthase (PGES). BK stimulated PGE(2) release in cultured HPASMC was inhibited by the PI 3-kinase inhibitor LY294002 and the p38 MAP kinase inhibitor SB202190. The inhibitory mechanism used by LY294002 did not involve cytosolic PLA(2) activation or COX-1, COX-2 and PGES protein expression but rather a novel effect on COX enzymatic activity. SB202190 also inhibited COX activity. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：30 / 34

页数：5

共 24 条

[1] PRODUCTION OF PGE(2) BY BOVINE CULTURED AIRWAY SMOOTH-MUSCLE CELLS - REGULATION BY CAMP [J].

BARRY, T ;

DELAMERE, F ;

HOLLAND, E ;

PAVORD, I ;

KNOX, A .

JOURNAL OF APPLIED PHYSIOLOGY, 1995, 78 (02) :623-628

[2]

Bishop-Bailey D, 1999, INT J MOL MED, V3, P41

[3] Effect of bradykinin, TGF-β1, IL-1β, and hypoxia on COX-2 expression in pulmonary artery smooth muscle cells [J].

Bradbury, DA ;

Newton, R ;

Zhu, YM ;

Stocks, J ;

Corbett, L ;

Holland, ED ;

Pang, LH ;

Knox, AJ .

AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2002, 283 (04) :L717-L725

[4] COX-3, a cyclooxygenase-1 variant inhibited by acetaminophen and other analgesic/antipyretic drugs: Cloning, structure, and expression [J].

Chandrasekharan, NV ;

Dai, H ;

Roos, KLT ;

Evanson, NK ;

Tomsik, J ;

Elton, TS ;

Simmons, DL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (21) :13926-13931

[5] Phosphoinositide 3-kinase: A critical signalling event in pulmonary cells [J].

Condliffe A.M. ;

Cadwallader K.A. ;

Walker T.R. ;

Rintoul R.C. ;

Cowburn A.S. ;

Chilvers E.R. .

Respiratory Research, 2000, 1 (1) :24-29

[6] PRODUCTION OF PGE(2) BY BOVINE CULTURED AIRWAY SMOOTH-MUSCLE CELLS AND ITS INHIBITION BY CYCLOOXYGENASE INHIBITORS [J].

DELAMERE, F ;

HOLLAND, E ;

PATEL, S ;

BENNETT, J ;

PAVORD, I ;

KNOX, A .

BRITISH JOURNAL OF PHARMACOLOGY, 1994, 111 (04) :983-988

[7] The growing phospholipase A(2) superfamily of signal transduction enzymes [J].

Dennis, EA .

TRENDS IN BIOCHEMICAL SCIENCES, 1997, 22 (01) :1-2

[8] Human glutathione dependent prostaglandin E synthase:: gene structure and regulation [J].

Forsberg, L ;

Leeb, L ;

Thorén, S ;

Morgenstern, R ;

Jakobsson, PJ .

FEBS LETTERS, 2000, 471 (01) :78-82

[9] MAPK regulation of gene expression in airway smooth muscle [J].

Gerthoffer, WT ;

Singer, CA .

RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY, 2003, 137 (2-3) :237-250

[10] Up-regulation of prostaglandin E2 synthesis by interleukin-1β in human orbital fibroblasts involves coordinate induction of prostaglandin-endoperoxide H synthase-2 and glutathione-dependent prostaglandin E2 synthase expression [J].

Han, R ;

Tsui, SL ;

Smith, TJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (19) :16355-16364

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