Diversity of gut Bifidobacterium species is not altered between allergic and non-allergic French infants

被引:21
作者
Waligora-Dupriet, A. J. [1 ]
Campeotto, F. [1 ,2 ]
Romero, K. [1 ]
Mangin, I. [6 ]
Rouzaud, G. [1 ]
Menard, O. [1 ]
Suau, A. [6 ]
Soulaines, P. [2 ]
Nicolis, I. [3 ,4 ]
Kapel, N. [1 ,5 ]
Dupont, C. [2 ]
Butel, M. J. [1 ]
机构
[1] Univ Paris 05, Fac Sci Pharmaceut & Biol, EA 4065, F-75006 Paris, France
[2] Univ Paris 05, Grp Hosp Cochin St Vincent de Paul, APHP, F-75006 Paris, France
[3] Univ Paris 05, Fac Sci Pharmaceut & Biol, EA 4466, F-75006 Paris, France
[4] Univ Paris 05, Fac Sci Pharmaceut & Biol, Dept Sante Publ & Biostat, F-75006 Paris, France
[5] Grp Hosp Pitie Salpetriere, APHP, F-75634 Paris, France
[6] Conservatoire Natl Arts & Metiers, Lab Biol EA 3199, Paris, France
关键词
Bifidobacterium; Diversity; BOX-PCR; Infants; Allergy; INTESTINAL MICROBIOTA; FECAL MICROBIOTA; CLOSTRIDIUM-DIFFICILE; ATOPIC ECZEMA; CHILDREN; IDENTIFICATION; MICROFLORA; HEALTHY; FLORA; PCR;
D O I
10.1016/j.anaerobe.2011.04.003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Some clinical studies have suggested a relationship between allergic diseases and gut microbiota. We aimed to study bifidobacterial colonization at species and strain levels in ten allergic French infants included at their first clinical consultation and 20 controls matching for age at sampling, mode of delivery, per partum antibiotics, type of feeding and antibiotics in the first weeks of life. The faecal microbiota was analyzed by culture methods and TTGE. Bifidobacterial species and strains were identified using multiplex PCR and Box-PCR fingerprinting. No differences were observed between groups in the number of colonized infants or in the levels of colonization by the main aerobic and anaerobic genera. All infants were colonized with high levels of Bifidobacterium except for one in each group. One to 5 Bifidobacterium species and 1 to 7 strains were observed per subject independently of allergic status and age at sampling. Our study showed the infants to be colonized by several species and strains, including several strains from the same species. This diversity in Bifidobacterium colonization was not related with the allergic status and showed that the link between Bifidobacterium colonization and allergic diseases is complex and cannot be restricted to the role attributed to Bifidobacterium species. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:91 / 96
页数:6
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