Assessing genetic structure and diversity of airborne bacterial communities by DNA fingerprinting and 16S rDNA clone library

被引:139
作者
Maron, PA
Lejon, DPH
Carvalho, E
Bizet, K
Lemanceau, P
Ranjard, L
Mougel, C
机构
[1] Univ Bourgogne, UMR Microbiol & Geochim Sols, INRA, CMSE, F-21065 Dijon, France
[2] Bourgogne Technol, F-21017 Dijon, France
[3] Bertin Technol, F-78180 Montigny Bretonneaux, France
关键词
bacteria; air; diversity; community structure;
D O I
10.1016/j.atmosenv.2005.03.002
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The density, genetic structure and diversity of airborne bacterial communities were assessed in the outdoor atmosphere. Two air samples were collected on the same location (north of France) at two dates (March 2003 (sample1) and May 2003 (sample 2)). Molecular culture-independent methods were used to characterise airborne bacterial communities regardless of the cell culturability. The automated-ribosomal intergenic spacer analysis (A-RISA) was performed to characterise the community structure in each sample. For both sampling dates, complex A-RISA patterns were observed suggesting a highly diverse community structure, comparable to those found in soil, water or sediment environments. Furthermore, differences in the genetic structure of airborne bacterial communities were observed between samples 1 and 2 suggesting an important variability in time. A clone library of 16S rDNA directly amplified from air DNA of sample I was constructed and sequenced to analyse the community composition and diversity. The Proteobacteria group had the greatest representation (60%), with bacteria belonging to the different subdivisions alpha-(19%), beta-(21%), gamma-(12%) and delta-(8%). Firmicute and Actinobacteria were also well represented with 14% and 12%, respectively. Most of the identified bacteria are known to be commonly associated with soil or plant environments suggesting that the atmosphere is mainly colonised transiently by microorganisms from local sources, depending on air fluxes. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3687 / 3695
页数:9
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