Purification of pectinases by three-phase partitioning

被引：46

作者：

Sharma, A ^{[1
]}

Gupta, MN ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, New Delhi 110016, India

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 19期

关键词：

Aspergillus niger; pectinase; protein precipitation; protein purification; three-phase partitioning; tomato pectinase;

D O I：

10.1023/A:1011984517432

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Three-phase partitioning was used to purify pectinases from Aspergillus niger and tomato by addition of tert-butanol in the presence of ammonium sulphate. The yields of 76% (Aspergillus niger) and 183% (tomato) and purifications of 10-fold (Aspergillus niger) and 9-fold (tomato) were obtained. The final purified enzyme enzyme from tomato showed a single band on SDS-PAGE with a molecular weight of 46 kDa.

引用

页码：1625 / 1627

页数：3

共 7 条

[1]

Industrial applications of pectic enzymes: a review [J].

Alkorta, I ;

Garbisu, C ;

Llama, MJ ;

Serra, JL .

PROCESS BIOCHEMISTRY, 1998, 33 (01) :21-28

[2]

Three phase partitioning: Concentration and purification of proteins [J].

Dennison, C ;

Lovrien, R .

PROTEIN EXPRESSION AND PURIFICATION, 1997, 11 (02) :149-161

[3]

MAN B, 1996, FOOD CHEM, V56, P433

[4]

CARBOHYDRATE-COMPOSITION AND ELECTROPHORETIC PROPERTIES OF TOMATO POLYGALACTURONASE ISOENZYMES [J].

MOSHREFI, M ;

LUH, BS .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1983, 135 (03) :511-514

[5]

A NEW METHOD FOR IMMOBILIZATION OF PECTIC ENZYMES [J].

PIFFERI, PG ;

SPAGNA, G ;

RINCON, RN ;

SETTI, L .

BIOTECHNOLOGY TECHNIQUES, 1993, 7 (06) :457-460

[6]

Purification of alkaline phosphatase from chicken intestine by three-phase partitioning and use of phenyl-Sepharose 6B in the batch mode [J].

Sharma, A ;

Sharma, S ;

Gupta, MN .

BIOSEPARATION, 2000, 9 (03) :155-161

[7]

Enhancement of enzyme activity through three-phase partitioning:: crystal structure of a modified serine proteinase at 1.5 Å resolution [J].

Singh, RK ;

Gourinath, S ;

Sharma, S ;

Roy, I ;

Gupta, MN ;

Betzel, C ;

Srinivasan, A ;

Singh, TP .

PROTEIN ENGINEERING, 2001, 14 (05) :307-313

← 1 →