Methodology to label mixed carbohydrate components by APTS

被引：19

作者：

Bui, Annamaria ^{[1
]}

Kocsis, Bela ^{[2
]}

Kilar, Ferenc ^{[1
,3
]}

机构：

[1] Univ Pecs, Fac Med, Inst Bioanal, H-7624 Pecs, Hungary

[2] Univ Pecs, Fac Med, Dept Med Microbiol & Immunol, H-7624 Pecs, Hungary

[3] Univ Pecs, Fac Sci, Dept Analyt & Environm Chem, H-7624 Pecs, Hungary

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2008年 / 70卷 / 06期

关键词：

capillary electrophoresis; carbohydrates; fluorescent labelling; APTS;

D O I：

10.1016/j.jprot.2008.01.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Labelling of carbohydrates with fluorescent dyes is of importance in the analysis of complex oligo- or polysaccharides. The complexity of the hydrolization mixtures of the oligosaccharides can be studied with separation methods after labelling. However, the procedures might provide unexpected phenomena. The experimental circumstances for labelling carbohydrates with 8-aininopyrene-1,3,6-trisulfonic acid were studied and the labelling efficiency of mono and oligosaccharides present in endotoxins was followed by capillary electrophoresis using LIF detection. Significant differences were observed in the labelling of the different sugar molecules, and the lowest reactivity with the dye was observed in the cases of amino-sugars. The results provide a basis for studies in determining the structures of the core parts and the O-chains of bacterial endotoxins. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：1313 / 1316

页数：4

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