DNA-binding specificity, transcriptional activation potential, and the rin mutation effect for the tomato fruit-ripening regulator RIN

被引：199

作者：

Ito, Yasuhiro ^{[1
]}

Kitagawa, Mamiko ^{[2
]}

Ihashi, Nao ^{[1
]}

Yabe, Kimiko ^{[1
]}

Kimbara, Junji ^{[2
]}

Yasuda, Junichi ^{[1
]}

Ito, Hirotaka ^{[2
]}

Inakuma, Takahiro ^{[2
]}

Hiroi, Seiji ^{[2
]}

Kasumi, Takafumi ^{[3
]}

机构：

[1] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

[2] Kagome Co Ltd, Res Inst, Nasushiobara, Tochigi 3292762, Japan

[3] Nihon Univ, Dept Agr & Biol Chem, Kanagawa 2528510, Japan

来源：

PLANT JOURNAL | 2008年 / 55卷 / 02期

关键词：

MADS box; rin mutant; DNA binding; fruit ripening; SEPALLATA; tomato;

D O I：

10.1111/j.1365-313X.2008.03491.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The RIN gene encodes a putative MADS box transcription factor that controls tomato fruit ripening, and its ripening inhibitor (rin) mutation yields non-ripening fruit. In this study, the molecular properties of RIN and the rin mutant protein were clarified. The results revealed that the RIN protein accumulates in ripening fruit specifically and is localized in the nucleus of the cell. In vitro studies revealed that RIN forms a stable homodimer that binds to MADS domain-specific DNA sites. Analysis of binding site selection experiments revealed that the consensus binding sites of RIN highly resemble those of the SEPALLATA (SEP) proteins, which are Arabidopsis MADS box proteins that control the identity of floral organs. RIN exhibited a transcription-activating function similar to that exhibited by the SEP proteins. These results indicate that RIN exhibits similar molecular functions to SEP proteins although they play distinctly different biological roles. In vivo assays revealed that RIN binds to the cis-element of LeACS2. Our results also revealed that the rin mutant protein accumulates in the mutant fruit and exhibits a DNA-binding activity similar to that exhibited by the wild-type protein, but has lost its transcription-activating function, which in turn would inhibit ripening in mutant fruit.

引用

页码：212 / 223

页数：12

共 49 条

[41] Binding site selection for the plant MADS domain protein AGL15 -: An in vitro and in vivo study [J].