Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors

被引：191

作者：

Ai, Hui-wang ^{[1
]}

Hazelwood, Kristin L. ^{[2
,3
]}

Davidson, Michael W. ^{[2
,3
]}

Campbell, Robert E. ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

[2] Florida State Univ, Natl High Magnet Field Lab, Tallahassee, FL 32310 USA

[3] Florida State Univ, Dept Biol Sci, Tallahassee, FL 32310 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 05期

关键词：

D O I：

10.1038/NMETH.1207

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence resonance energy transfer (FRET) with fluorescent proteins is a powerful method for detection of protein-protein interactions, enzyme activities and small molecules in the intracellular milieu. Aided by a new violet-excitable yellow-fluorescing variant of Aequorea victoria GFP, we developed dual FRET-based caspase-3 biosensors. Owing to their distinct excitation profiles, each FRET biosensor can be ratiometrically imaged in the presence of the other.

引用

页码：401 / 403

页数：3

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