Separation of complex branched polymers by size-exclusion chromatography probed with multiple detection

被引：68

作者：

Gaborieau, Marianne ^{[1
,2
]}

Nicolas, Julien ^{[3
]}

Save, Maud ^{[3
]}

Charleux, Bernadette ^{[3
]}

Vairon, Jean-Pierre ^{[3
]}

Gilbert, Robert G. ^{[1
]}

Castignolles, Patrice ^{[1
,3
]}

机构：

[1] Univ Queensland, Ctr Nutr & Food Sci, Brisbane, Qld 4072, Australia

[2] Max Planck Inst Polymer Res, D-55128 Mainz, Germany

[3] Univ Paris 06, Lab Chim Polymers, UMR 7610, F-75252 Paris 05, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2008年 / 1190卷 / 1-2期

基金：

澳大利亚研究理事会;

关键词：

multiple-detection size-exclusion chromatography (SEC); separation; polyacrylate; complex branched polymer; molecular weight; hydrodynamic volume;

D O I：

10.1016/j.chroma.2008.03.031

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Size-exclusion chromatography (SEC) separates polymers by hydrodynamic volume (the universal calibration principle). Molecular weights can be determined using viscometry (relying on universal calibration) and light scattering (independent of universal calibration). In the case of complex branched polyacrylates with tetrahydrofuran as eluent, universal calibration is valid, although the separation in term of molecular weight is incomplete: a given elution slice contains a range of molecular weights, described in terms of a 'local polydispersity'. The local polydispersity index decreases when the number of branches per chain increases and complete separation is reached for highly branched chains. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.

引用

页码：215 / 223

页数：9

共 81 条

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