Correlation between cell morphology and aggrecan gene expression level during differentiation from mesenchymal stem cells to chondrocytes

被引：22

作者：

Takagi, Mutsumi ^{[1
]}

Kitabayashi, Takayuki ^{[1
]}

Koizumi, Satoru ^{[1
]}

Hirose, Haruka ^{[1
]}

Kondo, Shin-ichi ^{[1
]}

Fujiwara, Masashi ^{[1
]}

Ueno, Kosei ^{[2
]}

Hiroaki, Misawa ^{[2
]}

Hosokawa, Yoichiroh ^{[3
,4
]}

Masuhara, Hiroshi ^{[3
,4
]}

Wakitani, Shigeyuki ^{[5
]}

机构：

[1] Hokkaido Univ, Grad Sch Engn, Div Biotechnol & Macromol Chem, Kita Ku, Sapporo, Hokkaido 0608628, Japan

[2] Hokkaido Univ, Res Inst Elect Sci, Sect Informat & Proc, Kita Ku, Sapporo, Hokkaido 0010021, Japan

[3] Osaka Univ, Dept Appl Phys, Frontier Res Ctr, Suita, Osaka 5650871, Japan

[4] Osaka Univ, Venture Business Lab, Suita, Osaka 5650871, Japan

[5] Osaka City Univ, Sch Med, Dept Orthoped Surg, Abeno Ku, Osaka, Osaka 5458585, Japan

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 07期

关键词：

aggrecan; differentiation; laser; mesenchymal stem cell; morphology; noninvasive;

D O I：

10.1007/s10529-008-9683-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 mu m(2) and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast-like to polygonal and the percentage of the large polygonal cells increased almost in proportion to aggrecan mRNA expression level during the differentiation culture of mesenchymal stem cells (MSCs) to chondrocytes. Approximately 80% of the large polygonal cells were negative for MSC marker (CD90, CD166) expression and the aggrecan mRNA expression level of the large polygonal cells was markedly higher than that of cells with other morphologies.

引用

页码：1189 / 1195

页数：7

共 12 条

[1]

Mesenchymal stem cells in perichondrium express activated leukocyte cell adhesion molecule and participate in bone marrow formation [J].