Rapid measurement of total antioxidant capacity in plants

被引：195

作者：

Gillespie, Kelly M. ^{[1
]}

Chae, June M. ^{[2
]}

Ainsworth, Elizabeth A. ^{[1
,2
]}

机构：

[1] Univ Illinois, Inst Genom Biol, Program Physiol & Mol Plant Biol, Urbana, IL 61801 USA

[2] USDA ARS, Photosynthes Res Unit, Urbana, IL 61801 USA

来源：

NATURE PROTOCOLS | 2007年 / 2卷 / 04期

关键词：

D O I：

10.1038/nprot.2007.100

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

There is growing interest in measuring the antioxidant status of plant tissues. This protocol describes the oxygen radical absorbance capacity (ORAC) assay, which measures antioxidant inhibition of peroxyl radical-induced oxidations and is a measure of total antioxidant capacity. The assay is performed in a microplate and is assessed with a 96-well multi-detection plate reader. Total antioxidant capacity of 64 experimental samples can easily be analyzed in 1 d. This assay is presented along with rapid assays for total phenolic content and total ascorbate content. Overall, these assays provide a general diagnostic tool of the antioxidant capacity in leaf tissue extracts.

引用

页码：867 / 870

页数：4

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