Evaluation of pentaproline-based chiral stationary phase by LC

被引：6

作者：

Bao, Ye ^{[2
]}

Huang, Junmin ^{[2
]}

Li, Tingyu ^{[1
]}

Armstrong, Daniel W. ^{[2
]}

机构：

[1] Mississippi State Univ, Dept Chem, Mississippi State, MS 39762 USA

[2] Univ Texas Arlington, Dept Chem & Biochem, Arlington, TX 76019 USA

来源：

CHROMATOGRAPHIA | 2008年 / 67卷 / Suppl 1期

关键词：

column liquid chromatography; enantiomeric separation; chiral stationary phase; peptide selector; proline;

D O I：

10.1365/s10337-007-0438-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A pentaproline-based chiral stationary phase was prepared and the selectivity of the column was evaluated with 194 racemic compounds in three mobile phase modes: normal-phase mode, polar organic mode and reversed-phase mode. 94 racemates out of 194 were separated and the normal-phase mode proved to be the separation mode of broadest applicability. The column is stable in all common organic solvents and no degradation in column performance was observed in any mode even after more than 1,000 injections. A brief sample loading test was performed on the 250 mm x 4.6 mm column and 13.2 mg of alpha-methyl-9-anthracenemethanol was baseline separated. Retention behavior in the normal-phase mode and the effect of analyte structure on retention and enantioselectivity are discussed.

引用

页码：S13 / S32

页数：20

共 31 条

[1] GRADIENT LC SEPARATION OF MACROMOLECULES - THEORY AND MECHANISM [J].

ARMSTRONG, DW ;

BOEHM, RE .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1984, 22 (09) :378-385

[2] SURFACE SILANOLS IN SILICA-BONDED HYDROCARBONACEOUS STATIONARY PHASES .2. IRREGULAR RETENTION BEHAVIOR AND EFFECT OF SILANOL MASKING [J].

BIJ, KE ;

HORVATH, C ;

MELANDER, WR ;

NAHUM, A .

JOURNAL OF CHROMATOGRAPHY, 1981, 203 (JAN) :65-84

[3] MECHANISTIC CONSIDERATIONS ON THE REVERSED PHASE LIQUID-CHROMATOGRAPHIC SEPARATION OF PROTEINS [J].

BLANQUET, RS ;

BUI, KH ;

ARMSTRONG, DW .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1986, 9 (09) :1933-1949

[4]

BLASCHKE G, 1979, ARZNEIMITTEL-FORSCH, V29-2, P1640

[5]

Bodanszky M., 1993, PRINCIPLES PEPTIDE S, V2nd

[6]

CREIGHTON T, 1993, STRUCTURES MOL PROPE

[7] CHIRAL SELECTORS WITH CHELATING PROPERTIES IN LIQUID-CHROMATOGRAPHY - FUNDAMENTAL REFLECTIONS AND SELECTIVE REVIEW OF RECENT DEVELOPMENTS [J].

DAVANKOV, VA .

JOURNAL OF CHROMATOGRAPHY A, 1994, 666 (1-2) :55-76

[8] Enantiomer separation by counter-current chromatography -: Optimisation and drawbacks in the use of L-proline derivatives as chiral selectors [J].

Delgado, B ;

Pérez, E ;

Santano, MC ;

Minguillón, C .

JOURNAL OF CHROMATOGRAPHY A, 2005, 1092 (01) :36-42

[9] Chiral separation by chromatographic and electromigration techniques.: A review [J].

Gübitz, G ;

Schmid, MG .

BIOPHARMACEUTICS & DRUG DISPOSITION, 2001, 22 (7-8) :291-336

[10]

Guebitz G., 1994, Journal of Chromatography A, V666, P91

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