Characterization and subgrouping of Campylobacter concisus strains using protein profiles, conventional biochemical testing and antibiotic susceptibility

被引:17
作者
Aabenhus, R
Permin, H
Andersen, LP
机构
[1] Natl Univ Hosp Copenhagen, Rigshosp, Dept Infect Control & Prevent 6701, DK-2100 Copenhagen, Denmark
[2] Natl Univ Hosp Copenhagen, Rigshosp, Dept Clin Microbiol, DK-2100 Copenhagen, Denmark
[3] Natl Univ Hosp Copenhagen, Bispebjerg Hosp, Dept Internal Med, DK-2100 Copenhagen, Denmark
关键词
Campylobacter concisus; characterization; protein profiles; antibiotic resistance;
D O I
10.1097/00042737-200510000-00003
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Objective To characterize and subgroup clinical strains of Campylobacter concisus isolated from patients with gastrointestinal disease. Methods A total of 109 C. concisus isolates from 98 patients obtained between June 1997 and December 1998 were analysed using protein profiles, conventional biochemical tube tests, ApiCampy, and susceptibility patterns by Neosensitabs and E-test. Results Two groups were identified by using protein profiles. One resembled the ATCC 33237 type strain of oral origin, and a second group differing from it, particularly in the high molecular weight zone. Considerable diversity exists in the lower molecular range of the gels, also within assigned subgroups. Biochemical testing showed differences between the groups in the ability to reduce nitrate, ApiCampy testing also yielded differences between the two assigned groups, although reactions were highly heterogeneous. Resistance to erythromycin, ciprofloxacin, ampicillin, ceftriaxone and tetracycline occurred in 3%,13%, 7%, 11% and 0% of the isolates when using Neosensitabs. The E-test yielded comparable results 7%, 5%, 0%, 2% and 3%, respectively. Conclusion Results indicate that C. concisus can be assigned to two broad groups based on differences in protein profiles. No distinct phenotypic marker was identified. Susceptibility patterns are not suitable for discrimination between the two assigned groups. Further studies using a polyphasic approach including the application of genetic methods are needed to assess the complex taxonomy of this potential pathogen.
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页码:1019 / 1024
页数:6
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