Multicellular spheroids as an in vitro tumor model

被引:217
作者
Hamilton, G [1 ]
机构
[1] KH Lainz, Ludwig Boltzmann Inst Clin Oncol, A-1090 Vienna, Austria
关键词
multicellular spheroids; drug resistance; extracellular matrix; hyaluronidase; adhesion molecules; P-glycoprotein;
D O I
10.1016/S0304-3835(98)00198-0
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Multicellular spheroids (MCS) have been used as an in vitro model system of micrometastases and avascular tumor regions for studying cell adhesion-dependent resistance to cytotoxic drugs and possible reversal by chemosensitizers and adhesion-reversing agents. Multicellular drug resistance has been linked to limited accessibility of cell subpopulations, active drug efflux, quiescence of cells in deeper layers due to cell contact inhibition and adverse microenvironmental conditions like acidic extracellular pH, hypoxia and nutritional depletion. The shortcomings of MCS as a tumor model include limited knowledge of the mechanisms leading to necrosis/apoptosis of core cells, the production of an extracellular matrix (ECM) by tumor cells instead of intratumoral normal cell populations and the complex relationship of MCS parameters like size, growth regulation, synthesis of ECM components and others on the origin and pretreatment of the tumor cells and specific culture conditions. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:29 / 34
页数:6
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