Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana

被引：175

作者：

Kamauchi, S ^{[1
]}

Nakatani, H ^{[1
]}

Nakano, C ^{[1
]}

Urade, R ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Agr, Kyoto 6110011, Japan

来源：

FEBS JOURNAL | 2005年 / 272卷 / 13期

关键词：

endoplasmic reticulum; fluid microarray; gene expression; tunicamycin; unfolded protein response;

D O I：

10.1111/j.1742-4658.2005.04770.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Eukaryotic cells respond to the accumulation of unfolded proteins in the endoplasmic reticulum (ER). In this case, so-called unfolded protein response (UPR) genes are induced. We determined the transcriptional expression of Arabidopsis thaliana UPR genes by fluid microarray analysis of tunicamycin-treated plantlets. Two hundred and fifteen up-regulated genes and 17 down-regulated ones were identified. These genes were reanalyzed with functional DNA microarrays, using DNA fragments cloned through fluid microarray analysis. Finally, 36 up-regulated and two down-regulated genes were recognized as UPR genes. Among them, the up-regulation of genes related to protein degradation (HRD1, SEL-1L/HRD3 and DER1), regulation of translation (P58(IPK)), and apoptosis (BAX inhibitor-1) was reconfirmed by real-time reverse transcriptase-PCR. The induction of SEL-1L protein in an Arabidopsis membrane fraction on tunicamycin-treatment was demonstrated. Phosphorylation of initiation factor-2 alpha, which was inhibited by P58(IPK), was decreased in tunicamycin-treated plantlets. However, regulatory changes in translation caused by ER stress were not detected in Arabidopsis. Plant cells appeared to have a strategy for overcoming ER stress through enhancement of protein folding activity, degradation of unfolded proteins, and regulation of apoptosis, but not regulation of translation.

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页码：3461 / 3476

页数：16

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