Identification of androstenedione in a river containing paper mill effluent

被引：137

作者：

Jenkins, R

Angus, RA ^{[1
]}

McNatt, H

Howell, WM

Kemppainen, JA

Kirk, M

Wilson, EM

机构：

[1] Univ Alabama Birmingham, Dept Biol, Birmingham, AL 35294 USA

[2] Samford Univ, Dept Biol, Birmingham, AL 35229 USA

[3] Univ N Carolina, Reprod Biol Lab, Chapel Hill, NC 27599 USA

[4] Univ N Carolina, Dept Pediat, Chapel Hill, NC 27599 USA

[5] Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA

[6] Univ Alabama Birmingham, Comprehens Canc Ctr Mass Spectrometry Shared Faci, Birmingham, AL 35294 USA

来源：

ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY | 2001年 / 20卷 / 06期

关键词：

androgen receptor; environmental androgen; endocrine disruptor; mosquitofish; liquid chromatography-mass spectrometry;

D O I：

10.1002/etc.5620200622

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Effluent from a paper mill discharging into the Fenholloway River, Taylor County, Florida, USA, contains chemicals that masculinize females of the resident population of eastern mosquitofish (Gambusia holbrooki), as evidenced in females by elongated anal fins, which is normally a male-specific trait. To identify androgenic components in the effluent, water collected from the Fenholloway River and a control tributary was fractionated using solid-phase extraction and reverse-phase high-performance-liquid chromatography. Two Fenholloway River fractions induced androgen receptor-dependent transcriptional activity in transient transfection cell culture assays. Of these, androstenedione was confirmed by liquid chromatography-mass spectrometry with multiple reaction monitoring.

引用

页码：1325 / 1331

页数：7

共 46 条

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[Anonymous], 1962, ICHTHYOLOGY

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