Pathogenicity of SspI-positive infectious bursal disease virus and molecular characterization of the VP2 hypervariable region

被引:76
作者
Hoque, MM [1 ]
Omar, AR [1 ]
Chong, LK [1 ]
Hair-Bejo, M [1 ]
Aini, I [1 ]
机构
[1] Univ Putra Malaysia, Fac Vet Med, Serdang 43400, Selangor, Malaysia
关键词
D O I
10.1080/03079450120066377
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The pathogenicity of four isolates of infectious bursal disease virus (IBDV) that have restriction fragment length polymorphism patterns of very virulent IBDV (vvIBDV), based on the presence of SspI and TaqI sites in the VP2 hypervariable region, was studied in specific pathogen free chickens. Chickens inoculated with isolates 92/04, 94/B551 and 97/61 developed severe clinical signs with a high mortality ranging from 70 to 80%, whereas the 94/273 isolate caused 10% mortality. Regardless of the isolates, significant differences were noted in the bursal lesion scores and bursa: body weight ratio index in the infected groups in comparison with the control groups. However, the presence of lesions in non-bursal tissues, muscles, thymus and at the junction of the proventriculus and gizzard were found only in the 92/04, 97/61 and 94/B551 isolates. Restriction fragment length polymorphism and sequence analysis of the VP2 hypervariable region indicated that all the isolates can be classified as vvIBDV based on the presence of SspI and TaqI sites at nucleotide positions 1011 and 833, respectively. In addition, all the isolates had amino acid substitutions at P222A, V256I and L294I, which are characteristic for vvIBDV isolated from different parts of the world. All the isolates except 94/273 also had a StyI site at nucleotide position 888. The absence of a StyI site in this isolate was associated with amino acid substitution at 254 from G to S. The 94/273 also had an amino acid substitution at position 270 from A to E, which is variable in the STC, Cu1 and OH strains. The presence of amino acid substitutions from G254S and A270E in SspI- and TaqI-positive vvIBDV strains is very uncommon and has not been reported previously. These amino acid variations might have caused the 94/273 to become less virulent in specific pathogen free chickens and resemble a classical virulent IBDV strain.
引用
收藏
页码:369 / 380
页数:12
相关论文
共 36 条
[1]   THE CHARACTERIZATION AND MOLECULAR-CLONING OF THE DOUBLE-STRANDED-RNA GENOME OF AN AUSTRALIAN STRAIN OF INFECTIOUS BURSAL DISEASE VIRUS [J].
AZAD, AA ;
BARRETT, SA ;
FAHEY, KJ .
VIROLOGY, 1985, 143 (01) :35-44
[2]   A COMPARISON OF THE SEQUENCES OF SEGMENT-A OF 4 INFECTIOUS BURSAL DISEASE VIRUS-STRAINS AND IDENTIFICATION OF A VARIABLE REGION IN VP2 [J].
BAYLISS, CD ;
SPIES, U ;
SHAW, K ;
PETERS, RW ;
PAPAGEORGIOU, A ;
MULLER, H ;
BOURSNELL, MEG .
JOURNAL OF GENERAL VIROLOGY, 1990, 71 :1303-1312
[3]   VP2 SEQUENCES OF RECENT EUROPEAN VERY VIRULENT ISOLATES OF INFECTIOUS BURSAL DISEASE VIRUS ARE CLOSELY-RELATED TO EACH OTHER BUT ARE DISTINCT FROM THOSE OF CLASSICAL STRAINS [J].
BROWN, MD ;
GREEN, P ;
SKINNER, MA .
JOURNAL OF GENERAL VIROLOGY, 1994, 75 :675-680
[4]   Coding sequences of both genome segments of a European 'very virulunt' infectious bursal disease virus [J].
Brown, MD ;
Skinner, MA .
VIRUS RESEARCH, 1996, 40 (01) :1-15
[5]   Molecular characterization of seven Chinese isolates of infectious bursal disease virus: Classical, very virulent, and variant strains [J].
Cao, YC ;
Yeung, WS ;
Law, M ;
Bi, YZ ;
Leung, FC ;
Lim, BL .
AVIAN DISEASES, 1998, 42 (02) :340-351
[6]  
Chowdhury E. H., 1996, Asian-Australasian Journal of Animal Sciences, V9, P465
[7]   Antigenic and genetic relationships between European very virulent infectious bursal disease viruses and an early West African isolate [J].
Eterradossi, N ;
Arnauld, C ;
Tekaia, F ;
Toquin, D ;
Le Coq, H ;
Rivallan, G ;
Guittet, M ;
Domenech, J ;
van den Berg, TP ;
Skinner, MA .
AVIAN PATHOLOGY, 1999, 28 (01) :36-46
[8]  
Hair-Bejo M., 1993, Jurnal Veterinar Malaysia, V5, P49
[9]   SEQUENCE-ANALYSIS AND EXPRESSION OF THE HOST-PROTECTIVE IMMUNOGEN-VP2 OF A VARIANT STRAIN OF INFECTIOUS BURSAL DISEASE VIRUS WHICH CAN CIRCUMVENT VACCINATION WITH STANDARD TYPE-I STRAINS [J].
HEINE, HG ;
HARITOU, M ;
FAILLA, P ;
FAHEY, K ;
AZAD, A .
JOURNAL OF GENERAL VIROLOGY, 1991, 72 :1835-1843
[10]   GENOMIC STRUCTURE OF THE LARGE RNA SEGMENT OF INFECTIOUS BURSAL DISEASE VIRUS [J].
HUDSON, PJ ;
MCKERN, NM ;
POWER, BE ;
AZAD, AA .
NUCLEIC ACIDS RESEARCH, 1986, 14 (12) :5001-5012