Validation of an LDH assay for assessing nanoparticle toxicity

被引：207

作者：

Han, Xianglu ^{[1
]}

Gelein, Robert ^{[1
]}

Corson, Nancy ^{[1
]}

Wade-Mercer, Pamela ^{[1
]}

Jiang, Jingkun ^{[4
]}

Biswas, Pratim ^{[5
]}

Finkelstein, Jacob N. ^{[1
,2
,3
]}

Elder, Alison ^{[1
]}

Oberdoerster, Guenter ^{[1
]}

机构：

[1] Univ Rochester, Dept Environm Med, Rochester, NY 14627 USA

[2] Univ Rochester, Dept Pediat, Rochester, NY USA

[3] Univ Rochester, Dept Radiat Oncol, Rochester, NY USA

[4] Univ Minnesota, St Paul, MN USA

[5] Washington Univ St Louis, Dept Energy Environm & Chem Engn, St Louis, MO USA

来源：

TOXICOLOGY | 2011年 / 287卷 / 1-3期

关键词：

Nanoparticle; Titanium dioxide; LDH assay; Cytotoxicity; Dissolution assay; NADH; METAL-CATALYZED OXIDATION; WALLED CARBON NANOTUBES; LACTATE-DEHYDROGENASE; COPPER NANOPARTICLES; FIBER DISSOLUTION; IONIC-STRENGTH; PROTEINS; NADH;

D O I：

10.1016/j.tox.2011.06.011

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Studies showed that certain cytotoxicity assays were not suitable for assessing nanoparticle (NP) toxicity. We evaluated a lactate dehydrogenase (LDH) assay for assessing copper (Cu-40, 40 nm), silver (Ag-35, 35 nm; Ag-40, 40 nm), and titanium dioxide (TiO(2)-25, 25 nm) NPs by examining their potential to inactivate LDH and interference with beta-nicotinamide adenine dinucleotide (NADH), a substrate for the assay. We also performed a dissolution assay for some of the NPs. We found that the copper NPs, because of their high dissolution rate, could interfere with the LDH assay by inactivating LDH. Ag-35 could also inactivate LDH probably because of the carbon matrix used to cage the particles during synthesis. TiO(2)-25 NPs were found to adsorb LDH molecules. In conclusion, NP interference with the LDH assay depends on the type of NPs and the suitability of the assay for assessing NP toxicity should be examined case by case. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：99 / 104

页数：6

共 21 条

[1] The reliability and limits of the MTT reduction assay for carbon nanotubes-cell interaction [J].

Belyanskaya, Larisa ;

Manser, Plus ;

Spohn, Philipp ;

Bruinink, Arle ;

Wick, Peter .

CARBON, 2007, 45 (13) :2643-2648

[2] The role of poly(ethyleneimine) in stabilization against metal-catalyzed oxidation of proteins: a case study with lactate dehydrogenase [J].

Breccia, JD ;

Andersson, MM ;

Hatti-Kaul, R .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2002, 1570 (03) :165-173

[3] Adsorption of gases in multimolecular layers [J].

Brunauer, S ;

Emmett, PH ;

Teller, E .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1938, 60 :309-319

[4] Spectroscopic analysis confirms the interactions between single walled carbon nanotubes and various dyes commonly used to assess cytotoxicity [J].

Casey, A. ;

Herzog, E. ;

Davoren, M. ;

Lyng, F. M. ;

Byrne, H. J. ;

Chambers, G. .

CARBON, 2007, 45 (07) :1425-1432

[5] Detailed identification of plasma proteins adsorbed on copolymer nanoparticles [J].

Cedervall, Tommy ;

Lynch, Iseult ;

Foy, Martina ;

Berggard, Tord ;

Donnelly, Seamas C. ;

Cagney, Gerard ;

Linse, Sara ;

Dawson, Kenneth A. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (30) :5754-5756

[6] Role of stress in the self-limiting oxidation of copper nanoparticles [J].

Chen, CH ;

Yamaguchi, T ;

Sugawara, K ;

Koga, K .

JOURNAL OF PHYSICAL CHEMISTRY B, 2005, 109 (44) :20669-20672

[7] Acute toxicological effects of copper nanoparticles in vivo [J].

Chen, Z ;

Meng, HA ;

Xing, GM ;

Chen, CY ;

Zhao, YL ;

Jia, GA ;

Wang, TC ;

Yuan, H ;

Ye, C ;

Zhao, F ;

Chai, ZF ;

Zhu, CF ;

Fang, XH ;

Ma, BC ;

Wan, LJ .

TOXICOLOGY LETTERS, 2006, 163 (02) :109-120

[8] EFFECTS OF SELECTED CELL OSMOLYTES ON ACTIVITY OF LACTATE-DEHYDROGENASE FROM EURYHALINE POLYCHAETE, NEREIS-SUCCINEA [J].

CLARK, ME ;

ZOUNES, M .

BIOLOGICAL BULLETIN, 1977, 153 (03) :468-484

[9]

EASTES W, 1994, REGUL TOXICOL PHARM, V20, pS104

[10] Changes of phage T7 nucleoprotein structure at low ionic strength. A Raman spectroscopic study [J].

Grof, P ;

Aslanian, D ;

Ronto, G .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1996, 1289 (01) :95-104

← 1 2 3 →