Comprehensive analysis of a multidimensional liquid chromatography mass spectrometry dataset acquired on a quadrupole selecting, quadrupole collision cell, time-of-flight mass spectrometer - I. How much of the data is theoretically interpretable by search engines?

被引:41
作者
Chalkley, RJ
Baker, PR
Hansen, KC
Medzihradszky, KF
Allen, NP
Rexach, M
Burlingame, AL
机构
[1] Univ Calif San Francisco, Mass Spectrometry Facil, San Francisco, CA 94143 USA
[2] Stanford Univ, Dept Biol Sci, Stanford, CA 94305 USA
关键词
D O I
10.1074/mcp.D500001-MCP200
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
An in-depth analysis of a multidimensional chromatography-mass spectrometry dataset acquired on a quadrupole selecting, quadrupole collision cell, time-of-flight (QqTOF) geometry instrument was carried out. A total of 3269 CID spectra were acquired. Through manual verification of database search results and de novo interpretation of spectra 2368 spectra could be confidently determined as predicted tryptic peptides. A detailed analysis of the non-matching spectra was also carried out, highlighting what the non-matching spectra in a database search typically are composed of. The results of this comprehensive dataset study demonstrate that QqTOF instruments produce information-rich data of which a high percentage of the data is readily interpretable.
引用
收藏
页码:1189 / 1193
页数:5
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