Whole-field five-dimensional fluorescence microscopy combining lifetime and spectral resolution with optical sectioning

被引:43
作者
Siegel, J
Elson, DS
Webb, SED
Parsons-Karavassilis, D
Lévêque-Fort, S
Cole, MJ
Lever, MJ
French, PMW
Neil, MAA
Juskaitis, R
Sucharov, LO
Wilson, T
机构
[1] Univ London Imperial Coll Sci Technol & Med, Dept Phys, Femtosecond Opt Grp, London SW7 2BW, England
[2] Univ London Imperial Coll Sci Technol & Med, Dept Biol & Med Syst, London SW7 2BW, England
[3] Univ Oxford, Dept Engn Sci, Oxford OX1 3PJ, England
关键词
D O I
10.1364/OL.26.001338
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We report a novel whole-field three-dimensional fluorescence lifetime imaging microscope that incoporates multispectral imaging to provide five-dimensional (5-D) fluorescence microscopy, This instrument, which can acquire a 5-D data set in less than a minute, is based on potentially compact and inexpensive diode-pumped solid-state laser technology. We demonstrate that spectral discrimination as well as optical sectioning minimize artifacts in lifetime determination and illustrate how spectral discrimination improves the lifetime contrast of biological tissue. (C) 2001 Optical Society of America.
引用
收藏
页码:1338 / 1340
页数:3
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