Lung fluorescence imaging to evaluate tumor metastasis induced by AQP5 expression in murine model

被引：18

作者：

Zhang, Zi-qiang ^{[1
]}

Zhu, Zhu-xian ^{[2
]}

Bai, Chun-xue ^{[3
]}

机构：

[1] Shandong Univ, Qianfoshan Hosp Shandong Prov, Dept Pulm Med, Jinan 250014, Peoples R China

[2] Shandong Univ, Qianfoshan Hosp Shandong Prov, Dept Nephropathy, Jinan 250014, Peoples R China

[3] Fudan Univ, Zhongshan Hosp, Dept Pulm Med, Shanghai 200032, Peoples R China

来源：

MEDICAL ONCOLOGY | 2012年 / 29卷 / 01期

关键词：

Lung imaging; Green fluorescence protein (GFP); Metastasis; Aquaporin (AQP); GENE-TRANSFER; THYMIDINE KINASE; CELL-GROWTH; MICE; TIME;

D O I：

10.1007/s12032-010-9788-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Our recent study, by up-regulation of AQP5 expression, showed enhanced proliferation and migration potential in lung cancer. However, so far none of the in vivo study of gene silencing of AQP5 has been tested. In this study, we tested roles of AQP5 on lung cancer metastasis potential by gene silencing of AQP5 in two lung cancer cell lines and tried to monitor lung metastases with EGFP marker. Lungs were imaged at different time points and allowed an accurate evaluation of tumor burden over time. Our results showed significantly decreased metastasis potential in AQP5 gene-silencing cells. Lung imaging confirmed the frequency of metastasis in mice. These data provide more evidence that AQP5 plays important roles in the metastasis potential of lung cancer. Lung fluorescence imaging provides rapid monitoring for tumor growth and metastasis, and it also offers quantitative and sensitive analysis of tumor growth and metastasis, compared to the traditional histology technique.

引用

页码：205 / 211

页数：7

共 27 条

[1]

Visualization of advanced human prostate cancer lesions in living mice by a targeted gene transfer vector and optical imaging [J].