Automated analysis of embryonic gene expression with cellular resolution in C-elegans

被引：148

作者：

Murray, John Isaac ^{[1
]}

Bao, Zhirong ^{[1
]}

Boyle, Thomas J. ^{[1
]}

Boeck, Max E. ^{[1
]}

Mericle, Barbara L. ^{[1
]}

Nicholas, Thomas J. ^{[1
]}

Zhao, Zhongying ^{[1
]}

Sandel, Matthew J. ^{[1
]}

Waterston, Robert H. ^{[1
]}

机构：

[1] Univ Washington, Sch Med, Dept Genome Sci, Seattle, WA 98195 USA

来源：

NATURE METHODS | 2008年 / 5卷 / 08期

关键词：

D O I：

10.1038/nmeth.1228

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a system that permits the automated analysis of reporter gene expression in Caenorhabditis elegans with cellular resolution continuously during embryogenesis. We demonstrate its utility by defining the expression patterns of reporters for several embryonically expressed transcription factors. The invariant cell lineage permits the automated alignment of multiple expression profiles, allowing direct comparison of the expression of different genes' reporters. We also used this system to monitor perturbations to normal development involving changes both in cell-division timing and in cell fate. Systematic application of this system could reveal the gene activity of each cell throughout development.

引用

页码：703 / 709

页数：7

共 28 条

[1] Gene expression during the life cycle of Drosophila melanogaster [J].

Arbeitman, MN ;

Furlong, EEM ;

Imam, F ;

Johnson, E ;

Null, BH ;

Baker, BS ;

Krasnow, MA ;

Scott, MP ;

Davis, RW ;

White, KP .

SCIENCE, 2002, 297 (5590) :2270-2275

[2] A fork head/HNF-3 homolog expressed in the pharynx and intestine of the Caenorhabditis elegans embryo [J].

Azzaria, M ;

Goszczynski, B ;

Chung, MA ;

Kalb, JM ;

McGhee, JD .

DEVELOPMENTAL BIOLOGY, 1996, 178 (02) :289-303

[3] Automated cell lineage tracing in Caenorhabditis elegans [J].

Bao, ZR ;

Murray, JI ;

Boyle, T ;

Ooi, SL ;

Sandel, MJ ;

Waterston, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (08) :2707-2712

[4] Composition and dynamics of the Caenorhabditis elegans early embryonic transcriptome [J].

Baugh, LR ;

Hill, AA ;

Slonim, DK ;

Brown, EL ;

Hunter, CP .

DEVELOPMENT, 2003, 130 (05) :889-900

[5] The homeodomain protein PAL-1 specifies a lineage-specific regulatory network in the C-elegans embryo [J].

Baugh, LR ;

Hill, AA ;

Claggett, JM ;

Hill-Harfe, K ;

Wen, JC ;

Slonim, DK ;

Brown, EL ;

Hunter, CP .

DEVELOPMENT, 2005, 132 (08) :1843-1854

[6] WormBase:: new content and better access [J].

Bieri, Tamberlyn ;

Blasiar, Darin ;

Ozersky, Philip ;

Antoshechkin, Igor ;

Bastiani, Carol ;

Canaran, Payan ;

Chan, Juancarlos ;

Chen, Nansheng ;

Chen, Wen J. ;

Davis, Paul ;

Fiedler, Tristan J. ;

Girard, Lisa ;

Han, Michael ;

Harris, Todd W. ;

Kishore, Ranjana ;

Lee, Raymond ;

McKay, Sheldon ;

Muller, Hans-Michael ;

Nakamura, Cecilia ;

Petcherski, Andrei ;

Rangarajan, Arun ;

Rogers, Anthony ;

Schindelman, Gary ;

Schwarz, Erich M. ;

Spooner, Will ;

Tuli, Mary Ann ;

Van Auken, Kimberly ;

Wang, Daniel ;

Wang, Xiaodong ;

Williams, Gary ;

Durbin, Richard ;

Stein, Lincoln D. ;

Sternberg, Paul W. ;

Spieth, John .

NUCLEIC ACIDS RESEARCH, 2007, 35 :D506-D510

[7] AceTree:: a tool for visual analysis of Caenorhabditis elegans embryogenesis [J].

Boyle, Thomas J. ;

Bao, Zhirong ;

Murray, John I. ;

Araya, Carlos L. ;

Waterston, Robert H. .

BMC BIOINFORMATICS, 2006, 7 (1)

[8] A gene expression fingerprint of C-elegans embryonic motor neurons -: art. no. 42 [J].

Fox, RM ;

Von Stetina, SE ;

Barlow, SJ ;

Shaffer, C ;

Olszewski, KL ;

Moore, JH ;

Dupuy, D ;

Vidal, M ;

Miller, DM .

BMC GENOMICS, 2005, 6 (1)

[9] Patterns of gene expression during Drosophila mesoderm Development [J].

Furlong, EEM ;

Andersen, EC ;

Null, B ;

White, KP ;

Scott, MP .

SCIENCE, 2001, 293 (5535) :1629-1633

[10]

Hallam S, 2000, DEVELOPMENT, V127, P4239

← 1 2 3 →