Using aptamers to visualize and capture cancer cells

被引：45

作者：

Pu, Ying ^{[1
,3
,4
]}

Zhu, Zhi ^{[3
,4
]}

Liu, Huixia ^{[1
]}

Zhang, Jiani ^{[1
]}

Liu, Jun ^{[1
]}

Tan, Weihong ^{[2
,3
,4
]}

机构：

[1] Cent S Univ, Xiangya Hosp, Changsha 410008, Hunan, Peoples R China

[2] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Coll Biol, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China

[3] Univ Florida, Dept Chem, Ctr Res Bio Nano Interface, Shands Canc Ctr,UF Genet Inst, Gainesville, FL 32611 USA

[4] Univ Florida, McKnight Brain Inst, Gainesville, FL 32611 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2010年 / 397卷 / 08期

关键词：

Aptamer; Cell-SELEX; Cancer cells; Imaging; Visualization; Nanomaterials; Clinical impact; Detection and enrichment; FUNCTIONALIZED GOLD NANOPARTICLES; CIRCULATING TUMOR-CELLS; MOLECULAR RECOGNITION; COLORIMETRIC DETECTION; RNA-APTAMERS; EXPONENTIAL ENRICHMENT; IMMUNODEFICIENCY-VIRUS; REVERSE-TRANSCRIPTASE; MICROFLUIDIC CHAMBERS; SYSTEMATIC EVOLUTION;

D O I：

10.1007/s00216-010-3715-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Since diseased cells exist in exceedingly low concentration at the early stage of cancer, highly sensitive imaging and detection methods are required. By improving the methods for capturing and visualizing cancer cells, clinicians can diagnose metastatic relapse, stratify patients for therapeutic purposes, monitor response to drugs and therapies, and track tumor progression. Therefore, using advanced biotechnological and analytical methods combined with cell-SELEX (systematic evolution of ligands by exponential enrichment)-based aptamers, we improved the capture and visualization of diseased cells in a manner that is inexpensive, simple, sensitive, and fast. This multiplexed cancer detection platform therefore improves our control over a range of clinical exigencies, including cancer diagnosis, therapeutic modalities, and drug delivery systems.

引用

页码：3225 / 3233

页数：9

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