Dependency of γ-secretase complex activity on the structural integrity of the bilayer

被引：4

作者：

Zhou, Hua ^{[1
]}

Zhou, Shuxia ^{[1
]}

Walian, Peter J. ^{[1
]}

Jap, Bing K. ^{[1
]}

机构：

[1] Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Life Sci, Berkeley, CA 94720 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 402卷 / 02期

基金：

美国国家卫生研究院;

关键词：

Membrane protein; Aspartyl protease; Alzheimer's disease; LIPID RAFTS; INTRACELLULAR DOMAIN; INTRAMEMBRANE PROTEOLYSIS; SIGNAL-TRANSDUCTION; HUMAN BRAIN; BETA-APP; IN-VIVO; PRESENILIN; CLEAVAGE; RECONSTITUTION;

D O I：

10.1016/j.bbrc.2010.10.017

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

gamma-secretase is a membrane protein complex associated with the production of All peptides that are pathogenic in Alzheimer's disease. We have characterized the activity of gamma-secretase complexes under a variety of detergent solubilization and reconstitution conditions, and the structural state of proteoliposomes by electron microscopy. We found that gamma-secretase activity is highly dependent on the physical state or integrity of the membrane bilayer - partial solubilization may increase activity while complete solubilization will abolish it. The activity of well-solubilized gamma-secretase can be restored to near native levels when properly reconstituted into a lipid bilayer environment. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：291 / 296

页数：6

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