Phase-contrast imaging of thin biomaterials

The necessity of information about the inner microscopical features of low absorbing materials is one of the most important goals in the structural research field. So Far, non destructive analysis have been performed using contact radiography giving the scope for great advances in the production and application of new materials. However. the nature of interaction. namely X-ray absorption, limited the observations only to materials having sufficient heavy elements content. The adoption of a different X-ray interaction with matter which involves refractive properties of materials is at the basis of phase-contrast imaging. The novel method allows the use of high X-ray energies. for a deeper penetration and a lower released dose, without losing any information on the nature of the sample. A demonstration study. performed at the third generation European Synchrotron Radiation Facility (ESRF)-Grenoble, to show the potential of the new technique applied to biomaterials characterization is presented here. The test samples are a commercial matrix barrier (GUIDOR) intended to aid the healing process after periodontal surgery and a hydroxyapatite thin slab originally deposited by plasma spray technique on a TA6V alloy substrate. Phase-contrast images showed significant advantages revealing features that have negligible absorption contrast. The technique can be successfully used for the characterization of biomaterials. (C) 2001 Elsevier Science Ltd. All rights reserved.