Preparation of uniform rich cholesterol unilamellar nanovesicles using CO2-expanded solvents

被引:52
作者
Cano-Sarabia, Mary [1 ,3 ]
Ventosa, Nora [1 ,3 ]
Sala, Santiago [1 ,3 ]
Patino, Cristina [2 ]
Arranz, Rocio [2 ]
Veciana, Jaunie [1 ,3 ]
机构
[1] CSIC, Inst Ciencia Mat Barcelona, Cerdanyola Del Valles 08193, Spain
[2] CSIC, Ctr Nacl Biotecnol, E-28049 Madrid, Spain
[3] CIBER BBN, Networking Ctr Bioengn Biomat & Nanomed, Cerdanyola Del Valles 08193, Spain
关键词
D O I
10.1021/la7032109
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Nanoscopic and uniform unilamellar vesicles, rich in cholesterol, have been prepared by a new procedure, named "DELOS-SUSP", which is based on the depressurization of a cholesterol solution in CO2-expanded acetone into an aqueous solution containing a surfactant. The CO2 is used here as a cosolvent medium, allowing the straightforward preparation of vesicular systems with controlled size distribution, uniform shape, and stability unachievable by conventional mixing technologies. The resulting nanoscopic vesicular systems dispersed in water were characterized using dynamic light scattering, potential, turbidity, and cryogenic transmission electron microscopy. The influence of operational parameters of this new methodology on the physicochemical characteristics of the vesicular systems is also reported.
引用
收藏
页码:2433 / 2437
页数:5
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