Riboflavin and photoproducts in MC3T3-E1 differentiation

被引：30

作者：

Chaves Neto, Antonio Hernandes ^{[1
]}

Yano, Claudia Lumy ^{[1
]}

Paredes-Gamero, Edgar Julian ^{[2
]}

Machado, Daisy ^{[1
]}

Justo, Giselle Zenker ^{[2
]}

Peppelenbosch, Maikel P. ^{[3
]}

Ferreira, Carmen Verissima ^{[1
]}

机构：

[1] Univ Estadual Campinas, UNICAMP, Dept Bioquim, Inst Biol, BR-13083970 Campinas, SP, Brazil

[2] Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo, Brazil

[3] Univ Groningen, Univ Med Ctr Groningen, Dept Cell Biol, NL-9713 AV Groningen, Netherlands

来源：

TOXICOLOGY IN VITRO | 2010年 / 24卷 / 07期

基金：

巴西圣保罗研究基金会;

关键词：

Ascorbic acid; Differentiation; beta-Glycerophosphate; Osteoblast; Riboflavin; Signaling; FOCAL ADHESION KINASE; BONE MASS; IN-VITRO; OSTEOBLAST PHENOTYPE; COLLAGEN-SYNTHESIS; CELL; EXPRESSION; GROWTH; OSTEOPROTEGERIN; PROLIFERATION;

D O I：

10.1016/j.tiv.2010.07.026

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 [卫生毒理学];

摘要：

Photoderivatives of riboflavin can modulate the proliferation and survival of cancer cells. In this work, we examined the influence of riboflavin and photoderivatives on osteoblast differentiation induced by ascorbic acid and beta-glycerophosphate. These compounds decreased the osteoblast proliferation, increased the alkaline phosphatase activity, promoted a reduction in matrix metalloproteinase-2 activity and the decreased in the OPG/RANKL ratio. The effects of flavins on osteoblasts were unrelated to the antioxidant activity of these compounds. The biological activity of osteogenic medium containing riboflavin and its photoderivatives involved the activation of different signaling pathways (AKT, FAK, CaMKII), caspases-3, -8 and -9, and up-regulation of the expression and/or stabilization of osteoblastic transcription factors (Runx2 and beta-catenin). These findings suggest a potential use of flavins as adjuvants to improve bone metabolism. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1911 / 1919

页数：9

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