Multiple human herpesvirus-8 infection

被引:32
作者
Beyari, MM
Hodgson, TA
Cook, RD
Kondowe, W
Molyneux, EM
Scully, CM
Teo, CG
Porter, SR
机构
[1] UCL, Eastman Inst Oral Hlth Care Sci, Dept Oral Med, London WC1X 8LD, England
[2] Publ Hlth Lab Serv, Cent Publ Hlth Lab, Div Virus Reference, London, England
[3] Univ Malawi, Coll Med, Dept Paediat, Blantyre, Malawi
关键词
D O I
10.1086/377504
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In Malawian patients with Kaposi sarcoma (KS) and their relatives, we investigated nucleotide-sequence variation in human herpesvirus-8 (HHV-8) subgenomic DNA, amplified from oral and blood samples by use of polymerase chain reaction. Twenty-four people had amplifiable HHV-8 DNA in >1 sample; 9 (38%) were seropositive for human immunodeficiency virus type 1, 21 (88%) were anti-HHV-8-seropositive, and 7 (29%) had KS. Sequence variation was sought in 3 loci of the HHV-8 genome: the internal repeat domain of open-reading frame (ORF) 73, the KS330 segment of ORF 26, and variable region 1 of ORF K1. Significant intraperson/intersample and intrasample sequence polymorphisms were observed in 14 people (60%). For 3 patients with KS, intraperson genotypic differences, arising from nucleotide sequence variations in ORFs 26 and K1, were found in blood and oral samples. For 2 other patients with KS and for 9 people without KS, intraperson genotypic and subgenotypic differences, originating predominantly from ORF K1, were found in oral samples; for the 2 patients with KS and for 4 individuals without KS, intrasample carriage of distinct ORF K1 sequences also were discernible. Our findings imply HHV-8 superinfection.
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收藏
页码:678 / 689
页数:12
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