Specific and independent recognition of U3 and U5 att sites by human immunodeficiency virus type 1 integrase in vivo

被引：63

作者：

Masuda, T

Kuroda, MJ

Harada, S

机构：

[1] Tokyo Med & Dent Univ, Div Med Res, Dept Immunotherapeut, Bunkyo Ku, Tokyo 113, Japan

[2] Kumamoto Univ, Sch Med, Dept Biodefense & Med Virol, Kumamoto, Japan

来源：

JOURNAL OF VIROLOGY | 1998年 / 72卷 / 10期

关键词：

D O I：

10.1128/JVI.72.10.8396-8402.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The retroviral attachment (att) sites at viral DNA ends are cis-acting regions essential for proviral integration. To investigate the sequence features of att important for human immunodeficiency virus type 1 (HIV-1) integration in vivo, we generated a series of 25 att mutants of HIV-1 by mutagenesis of the U3, U5, or both boundaries of att. Our results indicated that the terminal 11 or 12 bp of viral DNA are sufficient for specific recognition by HIV-1 integrase (IN) and suggested that IN might recognize each att site independently in vivo.

引用

收藏

页码：8396 / 8402

页数：7

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