Physiological analysis of bacterial mechanosensitive channels

被引：18

作者：

Booth, Ian R. ^{[1
]}

Edwards, Michelle D. ^{[1
]}

Black, Susan ^{[1
]}

Schumann, Ulrike ^{[1
]}

Bartlett, Wendy ^{[1
]}

Rasmussen, Tim ^{[1
]}

Rasmussen, Akiko ^{[1
]}

Miller, Samantha ^{[1
]}

机构：

[1] Univ Aberdeen, Sch Med Sci, Inst Med Sci, Aberdeen, Scotland

来源：

OSMOSENSING AND OSMOSIGNALING | 2007年 / 428卷

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

D O I：

10.1016/S0076-6879(07)28003-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacterial mechanosensitive (MS) channels play a significant role in protecting cells against hypoosmotic shock. Bacteria that have been diluted from high osmolarity medium into dilute solution are required to cope with sudden water influx associated with an osmotic imbalance equivalent to 10 to 14 atm. The cell wall is only poorly expansive and the cytoplasmic membrane even less so. Thus, swelling is not an option and the cell must rapidly eject solutes to diminish the osmotic gradient and thereby preserve structural integrity. This chapter describes cellular assays of MS channel function and their interpretation.

引用

页码：47 / 61

页数：15

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