Increases in weight of growth hormone-deficient and immunodeficient (lit/scid) dwarf mice after grafting of hGH-secreting, primary human keratinocytes

被引：15

作者：

Bellini, MH ^{[1
]}

Peroni, CN ^{[1
]}

Bartolini, P ^{[1
]}

机构：

[1] Univ Sao Paulo, IPEN CNEN, Dept Biotechnol, BR-05508900 Sao Paulo, Brazil

来源：

FASEB JOURNAL | 2003年 / 17卷 / 13期

关键词：

gene therapy; retroviral vector; in vivo secretion; GH circulatory level;

D O I：

10.1096/fj.03-0018fje

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Primary human keratinocytes, stably transduced with the human growth hormone (hGH) gene (under control of the retroviral LTR promoter) and selected via geneticin secreted as much as 7 mug hGH/10(6) cells/day. Their grafting onto immunodeficient dwarf mice (lit/scid) led to hGH levels in the circulation that did not go below 0.2-0.3 ng/ml during a 12 day period (peak value, 1.5 ng/ml at 4 h). This phenomenon was associated with a body weight increase of the grafted animals (0.060 g/animal/day) significantly higher (P<0.01) than that of controls (0.023 g/animal/day). This is the first report describing successful utilization of immunodeficient dwarf mice (lit/scid) in keratinocyte-based hGH gene therapy.

引用

页码：2322 / +

页数：15

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