Hydrogen peroxide generated by copper amine oxidase is involved in abscisic acid-induced stomatal closure in Vicia faba

被引：158

作者：

An, Zhenfeng ^{[1
]}

Jing, Wen ^{[1
]}

Liu, Youliang ^{[1
]}

Zhang, Wenhua ^{[1
]}

机构：

[1] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Coll Life Sci, Nanjing 210095, Peoples R China

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 2008年 / 59卷 / 04期

基金：

美国国家科学基金会;

关键词：

abscisic acid; calcium; copper amine oxidase; hydrogen peroxide; putrescine; stomatal closure; Vicia faba;

D O I：

10.1093/jxb/erm370

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

H2O2 is an essential signal in absicic acid (ABA)-induced stomatal closure. It can be synthesized by several enzymes in plants. In this study, the roles of copper amine oxidase (CuAO) in H2O2 production and stomatal closure were investigated. Exogenous ABA stimulated apoplast CuAO activity, increased H2O2 production and [Ca2+]cyt levels in Vicia faba guard cells, and induced stomatal closure. These processes were impaired by CuAO inhibitor(s). In the metabolized products of CuAO, only H2O2 could induce stomatal closure. By the analysis of enzyme kinetics and polyamine contents in leaves, putrescine was regarded as a substrate of CuAO. Putrescine showed similar effects with ABA on the regulation of H2O2 production, [Ca2+]cyt levels, as well as stomatal closure. The results suggest that CuAO in V. faba guard cells is an essential enzymatic source for H2O2 production in ABA-induced stomatal closure via the degradation of putrescine. Calcium messenger is an important intermediate in this process.

引用

页码：815 / 825

页数：11

共 49 条

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