Detection of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus in swine sera by enzyme-linked immunosorbent assay

被引：20

作者：

Takikawa, N

Kobayashi, S

Ide, S

Yamane, Y

Tanaka, Y

Yamagishi, H

机构：

[1] Res. Center for Veterinary Science, Kitasato Institute, Kitamoto

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 1996年 / 58卷 / 04期

关键词：

ELISA; porcine reproductive and respiratory syndrome;

D O I：

10.1292/jvms.58.355

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

We developed an enzyme-linked immunosorbent assay (ELISA) to detect antibodies against PRRS virus in swine sera. The ELISA antigen was prepared from MARC-145 cells infected with PRRS virus. The results from serial serum samples from experimentally infected and random swine indicated that the ELISA was more sensitive than indirect fluorescent and immunoperoxidase monolayer assays. Since the ELISA enables many sera to be simultaneously and rapidly tested, it was useful for detection antibodies against PRRS virus in swine sera.

引用

收藏

页码：355 / 357

页数：3

相关论文

共 9 条

[1]

ALBINA E, 1992, ANN RECH VET, V23, P167

[2]

CHRISTIANSON WT, 1992, AM J VET RES, V53, P485

[3] ENHANCED REPLICATION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME (PRRS) VIRUS IN A HOMOGENEOUS SUBPOPULATION OF MA-104 CELL-LINE [J].

KIM, HS ;

KWANG, J ;

YOON, IJ ;

JOO, HS ;

FREY, ML .

ARCHIVES OF VIROLOGY, 1993, 133 (3-4) :477-483

[4] ISOLATION AND SEROLOGICAL CHARACTERIZATION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME (PRRS) VIRUSES FROM PIGS WITH REPRODUCTIVE AND RESPIRATORY DISORDERS IN JAPAN [J].

MURAKAMI, Y ;

KATO, A ;

TSUDA, T ;

MOROZUMI, T ;

MIURA, Y ;

SUGIMURA, T .

JOURNAL OF VETERINARY MEDICAL SCIENCE, 1994, 56 (05) :891-894

[5] ISOLATION OF A LELYSTAD VIRUS-LIKE AGENT FROM BRITISH PIGS AND SCANNING ELECTRON-MICROSCOPY OF INFECTED MACROPHAGES [J].

PATON, DJ ;

BROWN, IH ;

SCOTT, AC ;

DONE, SH ;

EDWARDS, S .

VETERINARY MICROBIOLOGY, 1992, 33 (1-4) :195-201

[6] MYSTERY SWINE DISEASE IN THE NETHERLANDS - THE ISOLATION OF LELYSTAD VIRUS [J].

WENSVOORT, G ;

TERPSTRA, C ;

POL, JMA ;

TERLAAK, EA ;

BLOEMRAAD, M ;

DEKLUYVER, EP ;

KRAGTEN, C ;

VANBUITEN, L ;

DENBESTEN, A ;

WAGENAAR, F ;

BROEKHUIJSEN, JM ;

MOONEN, PLJM ;

ZETSTRA, T ;

DEBOER, EA ;

TIBBEN, HJ ;

DEJONG, MF ;

VANTVELD, P ;

GROENLAND, GJR ;

VANGENNEP, JA ;

VOETS, MT ;

VERHEIJDEN, JHM ;

BRAAMSKAMP, J .

VETERINARY QUARTERLY, 1991, 13 (03) :121-130

[7] COMPARISON BETWEEN RESULTS OF VIRUS NEUTRALIZATION TEST AND THOSE OF 2 ELISAS WHEN SCREENING FOR ANTIBODIES TO PSEUDORABIES VIRUS IN THAILAND [J].

WONGWATCHARADUMRONG, R ;

MORENOLOPEZ, J .

JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 1990, 37 (10) :760-766

[8] AN INDIRECT FLUORESCENT-ANTIBODY TEST FOR THE DETECTION OF ANTIBODY TO SWINE INFERTILITY AND RESPIRATORY SYNDROME VIRUS IN SWINE SERA [J].

YOON, IJ ;

JOO, HS ;

CHRISTIANSON, WT ;

KIM, HS ;

COLLINS, JE ;

MORRISON, RB ;

DIAL, GD .

JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 1992, 4 (02) :144-147

[9]

1991, VET REC, P370