Soy isoflavonoids exhibit in vitro biological activities of loop diuretics

被引:11
作者
Martínez, RM
Giménez, I
Lou, JM
Mayoral, JA
Alda, JO
机构
[1] Fac Med, Dept Physiol & Pharmacol, Zaragoza 50009, Spain
[2] Univ Zaragoza, Fac Sci, Dept Organ Chem, E-50009 Zaragoza, Spain
关键词
isoflavonoids; furosemide; Na+-K+-2Cl(-) cotransport; ion transport; isolated perfused kidney; kidney physiology; rats; loop diuretic;
D O I
10.1093/ajcn/68.6.1354S
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Intake of soyfoods containing isoflavonoids is related to positive effects on heart and kidney diseases. Urinary equol, a potent inhibitor of Na+-K+-2Cl(-) cotransport, originates from the metabolism of daidzein by intestinal bacteria. Loop diuretics (eg, furosemide), acting through inhibition of Na+-K+-2Cl(-) cotransport, are used to maintain adequate blood volume. In the present work, we compare isoflavonoids' inhibition of cotransport and effects on the function and hemodynamics of isolated perfused rat kidneys with those of furosemide. Equol [IC,, (half-maximal inhibitory concentration): 23.6 +/- 3.6 mu mol/L], genistein (IC50: 34.8 +/- 2.6 mu mol/L), and daidzein (IC50: 140.0 +/- 24 mu mol/L) inhibited bumetanide-sensitive rubidium uptake in LLC-PK1 cells. The ICS, of equol and genistein was close to that of furosemide (IC50: 10.3 +/- 2.7 mu mol/L). Furosemide, equol, and genistein stimulated water, sodium, and potassium excretion by isolated rat kidneys in the same temporal pattern. None of the isoflavonoids significantly increased the glomerular filtration rate, but genistein induced significant vasorelaxation. We conclude that isoflavonoids exhibit biological activities of furosemide in vitro, at concentrations similar to those reported for other in vitro effects. More research is needed to evaluate the participation of cotransport inhibition by isoflavonoids in the healthful effects claimed for soy intake.
引用
收藏
页码:1354S / 1357S
页数:4
相关论文
共 23 条
  • [1] AKIYAMA T, 1987, J BIOL CHEM, V262, P5592
  • [2] Purification and chemical characterization of a potent inhibitor of the Na-K-Cl cotransport system in rat urine
    Alda, JO
    Mayoral, JA
    Lou, M
    Gimenez, I
    Martinez, RM
    Garay, RP
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 221 (02) : 279 - 285
  • [3] Soybean isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal rhesus monkeys
    Anthony, MS
    Clarkson, TB
    Hughes, CL
    Morgan, TM
    Burke, GL
    [J]. JOURNAL OF NUTRITION, 1996, 126 (01) : 43 - 50
  • [4] IDENTIFICATION OF THE PHYTO-OESTROGEN 3',7-DIHYDROXYISOFLAVAN, AN ISOMER OF EQUOL, IN HUMAN-URINE AND COWS MILK
    BANNWART, C
    ADLERCREUTZ, H
    WAHALA, K
    KOTIAHO, T
    HESSO, A
    BRUNOW, G
    HASE, T
    [J]. BIOMEDICAL AND ENVIRONMENTAL MASS SPECTROMETRY, 1988, 17 (01): : 1 - 6
  • [5] BARTHELMEBS M, 1994, N-S ARCH PHARMACOL, V349, P209
  • [6] BOLESPONTO LL, 1990, CLIN PHARMACOKINET, V18, P381
  • [7] Brater D C, 1991, Drugs, V41 Suppl 3, P14
  • [8] INFLUENCE OF DIET ON LIPID ABNORMALITIES IN HUMAN RENAL-DISEASE
    DAMICO, G
    GENTILE, MG
    [J]. AMERICAN JOURNAL OF KIDNEY DISEASES, 1993, 22 (01) : 151 - 157
  • [9] CELL-VOLUME AND K+ TRANSPORT DURING DIFFERENTIATION OF MOUSE ERYTHROLEUKEMIA-CELLS
    DELPIRE, E
    GULLANS, SR
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 266 (02): : C515 - C523
  • [10] TYROSINE KINASE INHIBITORS SUPPRESS AGONIST-INDUCED CONTRACTION IN SMOOTH-MUSCLE
    DISALVO, J
    STEUSLOFF, A
    SEMENCHUK, L
    SATOH, S
    KOLQUIST, K
    PFITZER, G
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1993, 190 (03) : 968 - 974