Calcium-activated chloride channel-2 in human epithelia

被引：28

作者：

Connon, CJ ^{[1
]}

Yamasaki, K

Kawasaki, S

Quantock, AJ

Koizumi, N

Kinoshita, S

机构：

[1] Kyoto Prefectural Univ Med, Dept Ophthalmol, Kamigyo Ku, Kyoto 6020841, Japan

[2] Univ Wales Coll Cardiff, Sch Optometry & Vis Sci, Cardiff, S Glam, Wales

[3] Univ Wales Coll Cardiff, Cardiff Inst Tissue Engn & Repair, Cardiff, S Glam, Wales

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2004年 / 52卷 / 03期

关键词：

human; hCLCA2; epithelium; immunohistochemistry; electron microscopy;

D O I：

10.1177/002215540405200313

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Calcium-activated chloride channels (CLCAs) are a family of multifunctional proteins that are widely distributed in tissues. To investigate the distribution of human CLCA-2 (hCLCA2) in human epithelia at the light and electron microscopic levels, we raised a primary antibody against a synthetic polypeptide sequence from natural hCLCA2. Corneal, skin, vaginal, esophageal, and laryngeal epithelia were immunopositive for hCLCA2 at the cytosolic aspect of the basal cells adjacent to the basement membrane. Epithelia of stomach and small intestine showed no hCLCA2 immunoreactivity. This study reports the cellular distribution of hCLCA2 in human epithelia and suggests its possible involvement in epithelial stratification and cell-substrate adhesion.

引用

页码：415 / 418

页数：4

共 12 条

[1] The breast cancer β4 integrin and endothelial human CLCA2 mediate lung metastasis [J].

Abdel-Ghany, M ;

Cheng, HC ;

Elble, RC ;

Pauli, BU .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (27) :25438-25446

[2] DISTINCT FUNCTIONS FOR INTEGRINS ALPHA-3-BETA-1 IN FOCAL ADHESIONS AND ALPHA-6-BETA-4 BULLOUS PEMPHIGOID ANTIGEN IN A NEW STABLE ANCHORING CONTACT (SAC) OF KERATINOCYTES - RELATION TO HEMIDESMOSOMES [J].

CARTER, WG ;

KAUR, P ;

GIL, SG ;

GAHR, PJ ;

WAYNER, EA .

JOURNAL OF CELL BIOLOGY, 1990, 111 (06) :3141-3154

[3] Ca2+-activated Cl- channels -: Focus on "Molecular cloning and transmembrane structure of hCLCA2 from human lung, trachea, and mammary gland" [J].

Cuppoletti, J ;

Malinowska, DH .

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1999, 276 (06) :C1259-C1260

[4] POLARIZED INTEGRIN MEDIATES HUMAN KERATINOCYTE ADHESION TO BASAL LAMINA [J].

DELUCA, M ;

TAMURA, RN ;

KAJIJI, S ;

BONDANZA, S ;

ROSSINO, P ;

CANCEDDA, R ;

MARCHISIO, PC ;

QUARANTA, V .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (17) :6888-6892

[5] Ca2+-activated Cl- channels:: a newly emerging anion transport family [J].

Fuller, CM ;

Ji, HL ;

Tousson, A ;

Elble, RC ;

Pauli, BU ;

Benos, DJ .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2001, 443 (Suppl 1) :S107-S110

[6]

Gruber AD, 1999, AM J PHYSIOL-CELL PH, V276, pC1261

[7]

Gruber AD, 1999, CANCER RES, V59, P5488

[8] A SIMPLE AND EFFICIENT METHOD FOR DIRECT CLONING OF PCR PRODUCTS USING DDT-TAILED VECTORS [J].

HOLTON, TA ;

GRAHAM, MW .

NUCLEIC ACIDS RESEARCH, 1991, 19 (05) :1156-1156

[9] Isolation and characterization of a Ca2+-activated chloride channel from human corneal epithelium [J].

Itoh, R ;

Kawamoto, S ;

Miyamoto, Y ;

Kinoshita, S ;

Okubo, K .

CURRENT EYE RESEARCH, 2000, 21 (06) :918-925

[10] Molecular structure and physiological function of chloride channels [J].

Jentsch, TJ ;

Stein, V ;

Weinreich, F ;

Zdebik, AA .

PHYSIOLOGICAL REVIEWS, 2002, 82 (02) :503-568

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