Determination of mono- and disaccharides in milk and milk products by high-performance anion-exchange chromatography with pulsed amperometric detection

被引：61

作者：

Cataldi, TRI ^{[1
]}

Angelotti, M ^{[1
]}

Bianco, G ^{[1
]}

机构：

[1] Univ Basilicata, Dipartimento Chim, I-85100 Potenza, Italy

来源：

ANALYTICA CHIMICA ACTA | 2003年 / 485卷 / 01期

关键词：

monosaccharides; disaccharides; milk; dairy products; anion-exchange chromatography; amperometric detection;

D O I：

10.1016/S0003-2670(03)00405-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and sensitive liquid chromatographic method for the separation and quantification of mono- and disaccharides in raw- and processed-milk is described. Samples of cows', buffalos', sheeps' and goats' milk were analyzed upon clarification and appropriate dilution for the quantification of lactose, galactose, glucose and N-acetylglucosamine (GlcNAc). The separation was accomplished by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD), using a gold working electrode and dilute alkaline eluents modified by a millimolar concentration of barium acetate. The eluent composition employed was designed to provide optimum separation with respect to the selected sample, without interference from the matrix components. The analytical method was successfully employed for the determination of mono- and disaccharides naturally occurring in dairy milk, mozzarella cheese and whey samples, with high sensitivity and accuracy. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：43 / 49

页数：7

共 38 条

[1]

ANDREWS GR, 1984, J SOC DAIRY TECH, V37, P3

[2]

[Anonymous], **DROPPED REF**

[3]

BARKER R, 1990, METHOD ENZYMOL, V83, P58

[4] Release of galactose and N-acetylglucosamine during the storage of UHT milk [J].

Belloque, J ;

Villamiel, M ;

López-Fandiño, R ;

Olano, A .

FOOD CHEMISTRY, 2001, 72 (04) :407-412

[5]

Bettenbrock K, 1998, APPL ENVIRON MICROB, V64, P2013

[6]

BJORNDAL HAKAN, 1967, CAR BOHYD RES, V5, P433, DOI 10.1016/S0008-6215(00)81115-5

[7] Isocratic separations of closely-related mono- and disaccharides by high-performance anion-exchange chromatography with pulsed amperometric detection using dilute alkaline spiked with barium acetate [J].

Cataldi, TRI ;

Campa, C ;

Angelotti, M ;

Bufo, SA .

JOURNAL OF CHROMATOGRAPHY A, 1999, 855 (02) :539-550

[8] Role of barium ions in the anion-exchange chromatographic separation of carbohydrates with pulsed amperometric detection [J].

Cataldi, TRI ;

Campa, C ;

Margiotta, G ;

Bufo, SA .

ANALYTICAL CHEMISTRY, 1998, 70 (18) :3940-3945

[9] Method development for the quantitative determination of lactulose in heat-treated milks by HPAEC with pulsed amperometric detection [J].

Cataldi, TRI ;

Angelotti, M ;

Bufo, SA .

ANALYTICAL CHEMISTRY, 1999, 71 (21) :4919-4925

[10] Carbohydrate analysis by high-performance anion-exchange chromatography with pulsed amperometric detection: The potential is still growing [J].

Cataldi, TRI ;

Campa, C ;

De Benedetto, GE .

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 2000, 368 (08) :739-758

← 1 2 3 4 →