The translational cis-regulatory element of mammalian ribosomal protein mRNAs is recognized by the plant translational apparatus

被引:30
作者
Shama, S [1 ]
Meyuhas, O [1 ]
机构
[1] HEBREW UNIV JERUSALEM,SCH MED,INST BIOCHEM,DEPT DEV BIOCHEM,IL-91010 JERUSALEM,ISRAEL
来源
EUROPEAN JOURNAL OF BIOCHEMISTRY | 1996年 / 236卷 / 02期
关键词
translational control; ribosomal proteins; polysomes; wheat germ;
D O I
10.1111/j.1432-1033.1996.00383.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The translational efficiency of mammalian ribosomal protein mRNAs correlates with the growth status of the cells and its control is mediated through a 5' terminal oligopyrimidine tract (5' TOP) common to all these mRNAs. In the present study, we demonstrate that the plant translational apparatus, as represented by wheat-germ extract, discriminates against mammalian mRNAs containing this motif to the same extent as do quiescent mammalian cells. Moreover, mutations in the 5' TOP, which abolish the growth-dependent translational control of the respective mRNAs in mammalian cells, render these mRNAs refractory to discrimination in the plant cell-free system. This selective discrimination reflects neither the specific instability of 5' TOP-containing mRNAs during the incubation in vitro nor a lower competitive potential for the cap-binding protein. The lower in vitro translational efficiency of these mRNAs is an inherent feature which is independent of whether they were derived from polysomes or messenger ribonucleoprotein particles of the transfected mammalian cells. The conservation of the discriminatory property of the translational apparatus between the animal and plant kingdoms is discussed from mechanistic and evolutionary points of view.
引用
收藏
页码:383 / 388
页数:6
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