Isolation, characterization and phylogenetic analysis of Acidiphilium-like bacteria from acid mine drainage

被引：3

作者：

Zhang, Yanfei ^{[1
]}

Yang, Yu ^{[1
]}

Liu, Jianshe ^{[1
]}

Qiu, Guanzhou ^{[1
]}

机构：

[1] Cent S Univ, Sch Resources Proc & Bioengn, Changsha 410083, Hunan, Peoples R China

来源：

BIOHYDROMETALLURY: FROM THE SINGLE CELL TO THE ENVIRONMENT | 2007年 / 20-21卷

关键词：

Acidiphilium spp; isolation; marmatite-bioleaching; 16S rRNA; GyrB; Rep-PCR;

D O I：

10.4028/www.scientific.net/AMR.20-21.473

中图分类号：

TF [冶金工业];

学科分类号：

0806 ;

摘要：

The acidophilic heterotrophic bacteria Acidiphilium spp. were considered as a ministrant of Leptospirillum spp., Acidithiobacillus spp. and other autotrophic bacteria in acid mine drainage (AMD). 25 Acidiphilium-like bacterial strains were isolated from eight different mines in China and their physiology was characterized. Their marmatite-bioleaching ability has been investigated and compared with that of a mixed culture of Acidiphilium sp. and Acidithiobacillus ferrooxidans. The results indicated that some Acidiphilium-like strains exhibited a high marmatite-bioleaching ability, which was even higher than that of Acidithiobacillus ferrooxidans. The leaching efficiency was not higher in mixed than in pure culture. The phylogenetic relationship of the 25 Acidiphilium-like strains was analyzed by 16S rRNA sequencing, GyrB sequencing and rep-PCR genomic fingerprinting. The 25 strains and other eight species of Acidiphilium spp. were clustered into three groups.

引用

页码：473 / +

页数：2

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