An immunoassay for atrazine using tunable immunosorbent

被引：39

作者：

Kim, JY

Mulchandani, A ^{[1
]}

Chen, W

机构：

[1] Univ Calif Riverside, Dept Environm Chem & Engn, Riverside, CA 92521 USA

[2] Univ Calif Riverside, Environm Toxicol Grad Program, Riverside, CA 92521 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2003年 / 322卷 / 02期

关键词：

atrazine; immunoassay; immunosorbent; elastin; scAb;

D O I：

10.1016/j.ab.2003.08.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel, simple, economical, and environmentally friendly tunable immunosorbent-based immunoassay for sensitive and selective determination of atrazine is reported. Tunable immunosorbents consisting of a fusion between an elastin-like polypeptide made up of 77 repeating units of the pentapeptide VPGVG and a single-chain Fv of an anti-atrazine antibody were synthesized biologically and purified by temperature-triggered phase transition. A competitive immunoassay based on the competition of atrazine-horseradish peroxidase and atrazine was established with IC50 and lower detection limit of 0.16 and 0.01 ppb, respectively. Excellent recoveries (mean values ranging between 92 and 104%) were demonstrated in simulated atrazine-contaminated water samples. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：251 / 256

页数：6