STR typing of human telogen hairs - a new approach

被引：95

作者：

Hellmann, A ^{[1
]}

Rohleder, U ^{[1
]}

Schmitter, H ^{[1
]}

Wittig, M ^{[1
]}

机构：

[1] Bundeskriminalamt, Kriminaltech Inst, D-65193 Wiesbaden, Germany

来源：

INTERNATIONAL JOURNAL OF LEGAL MEDICINE | 2001年 / 114卷 / 4-5期

关键词：

short tandem repeat loci; STR; degraded DNA; telogen hairs;

D O I：

10.1007/s004140000175

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

A new approach to short tandem repeat (STR) typing of DNA extracted from telogen shed hairs is presented. Newly designed primer pairs with annealing positions close to the repeat units of the STR loci HUMFES, HUMTH01 and HUMTPOX were used for amplification. The typing results were compared to those obtained by the commonly used primer pairs by means of success rates. The primer pairs capable of producing very short amplicons (< 106 bp with HUMFES, < 86 bp with HUMTH01 and < 87 bp with HUMTPOX) described in this study significantly increased the success rates when typing telogen hairs.

引用

页码：269 / 273

页数：5

共 26 条

[1] THE ENZYMATIC-ACTIVITY OF PROTEINASE-K IS CONTROLLED BY CALCIUM
BAJORATH, J
HINRICHS, W
SAENGER, W
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 176 (02): : 441 - 447
[2] CAENAZZO L, 1994, ADV FORENSIC HAEMOGE, V5, P349
[3] Decorte R, 1996, ADV FOREN H, V6, P17
[4] FORENSIC APPLICATION OF DNA FINGERPRINTS
GILL, P
JEFFREYS, AJ
WERRETT, DJ
[J]. NATURE, 1985, 318 (6046) : 577 - 579
[5] DNA TYPING FROM SINGLE HAIRS
HIGUCHI, R
VONBEROLDINGEN, CH
SENSABAUGH, GF
ERLICH, HA
[J]. NATURE, 1988, 332 (6164) : 543 - 546
[6] HOCHMEISTER MN, 1995, J FORENSIC SCI, V40, P701
[7] Hochmeister MN, 1996, J FORENSIC SCI, V41, P155
[8] Evaluation of a decontamination protocol for hair shafts before mtDNA sequencing
Jehaes, E
Gilissen, A
Cassiman, JJ
Decorte, R
[J]. FORENSIC SCIENCE INTERNATIONAL, 1998, 94 (1-2) : 65 - 71
[9] Kline MC, 1998, J FORENSIC SCI, V43, P250
[10] Linch CA, 1998, J FORENSIC SCI, V43, P305

← 1 2 3 →