Sodium, Potassium-ATPases in algae and oomycetes

被引：28

作者：

Barrero-Gil, J ^{[1
]}

Garciadeblás, B ^{[1
]}

Benito, B ^{[1
]}

机构：

[1] Univ Politecn Madrid, Escuela Tecn Super Ingenieros Agron, Dept Biotecnol, Madrid, Spain

来源：

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES | 2005年 / 37卷 / 04期

关键词：

potassium; sodium; proton; ATPase; algae; oomycetes;

D O I：

10.1007/s10863-005-6637-x

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have investigated the presence of K+- transporting ATPases that belong to the phylogenetic group of animal Na+, K+- ATPases in the Pythium aphanidermatum Stramenopile oomycete, the Porphyra yezoensis red alga, and the Udotea petiolata green alga, by molecular cloning and expression in heterologous systems. PCR amplification and search in EST databases allowed one gene to be identified in each species that could encode ATPases of this type. Phylogenetic analysis of the sequences of these ATPases revealed that they cluster with ATPases of animal origin, and that the algal ATPases are closer to animal ATPases than the oomycete ATPase is. The P. yezoensis and P. aphanidermatum ATPases were functionally expressed in Saccharomyces cerevisiae and Escherichia coli alkali cation transport mutants. The aforementioned cloning and complementary searches in silicio for H+- and Na+, K+- ATPases revealed a great diversity of strategies for plasma membrane energization in eukaryotic cells different from typical animal, plant, and fungal cells.

引用

页码：269 / 278

页数：10

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