Functional analysis of human microtubule-based motor proteins, the kinesins and dyneins, in mitosis/cytokinesis using RNA interference

被引:332
作者
Zhu, CJ
Zhao, J
Bibikova, M
Leverson, JD
Bossy-Wetzel, E
Fan, JB
Abraham, RT
Jiang, W [1 ]
机构
[1] Burnham Inst, La Jolla, CA 92037 USA
[2] Illumina, San Diego, CA 92121 USA
[3] Abbott Labs, Abbott Pk, IL 60064 USA
关键词
D O I
10.1091/mbc.E05-02-0167
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Microtubule (MT)-based motor proteins, kinesins and dyneins, play important roles in multiple cellular processes including cell division. In this study, we describe the generation and use of an Escherichia coli RNase III-prepared human kinesin/dynein esiRNA library to systematically analyze the functions of all human kinesin/dynein MT motor proteins. Our results indicate that at least 12 kinesins are involved in mitosis and cytokinesis. Eg5 (a member of the kinesin-5 family), Kif2A (a member of the kinesin-13 family), and KifC1 (a member of the kinesin-14 family) are crucial for spindle formation; KifC1, MCAK (a member of the kinesin-13 family), CENP-E (a member of the kinesin-7 family), Kif14 (a member of the kinesin-3 family), Kif18 (a member of the kinesin-8 family), and Kid (a member of the kinesin-10 family) are required for chromosome congression and alignment; Kif4A and Kif4B (members of the kinesin-4 family) have roles in anaphase spindle dynamics; and Kif4A, Kif4B, MKLP1, and MKLP2 (members of the kinesin-6 family) are essential for cytokinesis. Using immunofluorescence analysis, time-lapse microscopy, and rescue experiments, we investigate the roles of these 12 kinesins in detail.
引用
收藏
页码:3187 / 3199
页数:13
相关论文
共 69 条
[1]   M phase phosphoprotein 1 is a human plus-end-directed kinesin-related protein required for cytokinesis [J].
Abaza, A ;
Soleilhac, JM ;
Westendorf, J ;
Piel, M ;
Crevel, I ;
Roux, A ;
Pirollet, F .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (30) :27844-27852
[2]   Aurora B regulates MCAK at the mitotic centromere [J].
Andrews, PD ;
Ovechkina, Y ;
Morrice, N ;
Wagenbach, M ;
Duncan, K ;
Wordeman, L ;
Swedlow, JR .
DEVELOPMENTAL CELL, 2004, 6 (02) :253-268
[3]   Xkid, a chromokinesin required for chromosome alignment on the metaphase plate [J].
Antonio, C ;
Ferby, I ;
Wilhelm, H ;
Jones, M ;
Karsenti, E ;
Nebreda, AR ;
Vernos, I .
CELL, 2000, 102 (04) :425-435
[4]   Phosphorylation by p34(cdc2) regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo [J].
Blangy, A ;
Lane, HA ;
dHerin, P ;
Harper, M ;
Kress, M ;
Nigg, EA .
CELL, 1995, 83 (07) :1159-1169
[5]   Survivin is required for stable checkpoint activation in taxol-treated HeLa cells [J].
Carvalho, A ;
Carmena, M ;
Sambade, C ;
Earnshaw, WC ;
Wheatley, SP .
JOURNAL OF CELL SCIENCE, 2003, 116 (14) :2987-2998
[6]   TOGp, the human homolog of XMAP215/Dis1, is required for centrosome integrity, spindle pole organization, and bipolar spindle assembly [J].
Cassimeris, L ;
Morabito, J .
MOLECULAR BIOLOGY OF THE CELL, 2004, 15 (04) :1580-1590
[7]   Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC [J].
Chan, GKT ;
Jablonski, SA ;
Sudakin, V ;
Hittle, JC ;
Yen, TJ .
JOURNAL OF CELL BIOLOGY, 1999, 146 (05) :941-954
[8]   Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1 [J].
Chan, GKT ;
Schaar, BT ;
Yen, TJ .
JOURNAL OF CELL BIOLOGY, 1998, 143 (01) :49-63
[9]   Zebrafish mitotic kinesin-like protein 1 (Mklp1) functions in embryonic cytokinesis [J].
Chen, MC ;
Zhou, Y ;
Detrich, HW .
PHYSIOLOGICAL GENOMICS, 2002, 8 (01) :51-66
[10]   Centromeres and kinetochores: From epigenetics to mitotic checkpoint signaling [J].
Cleveland, DW ;
Mao, YH ;
Sullivan, KF .
CELL, 2003, 112 (04) :407-421