The Efficiency of Proanthocyanidin in an Experimental Pulmonary Fibrosis Model: Comparison with Taurine

被引:31
作者
Agackiran, Yetkin [2 ]
Gul, Husamettin [3 ]
Gunay, Ersin [1 ]
Akyurek, Nalan [4 ]
Memis, Leyla [4 ]
Gunay, Sibel [5 ]
Sirin, Yusuf Sinan [6 ]
Ide, Tayfun [7 ]
机构
[1] Afyon Kocatepe Univ, Sch Med, Dept Chest Dis, TR-03200 Afyon, Turkey
[2] Ataturk Chest Dis & Thorac Surg Training & Res Ho, Dept Pathol, Ankara, Turkey
[3] Gulhane Mil Med Acad, Dept Pharmacol, Ankara, Turkey
[4] Gazi Univ, Fac Med, Dept Pathol, Ankara, Turkey
[5] Igdir State Hosp, Chest Dis Clin, Igdir, Turkey
[6] Mehmet Akif Ersoy Univ, Fac Vet Med, Dept Surg, Burdur, Turkey
[7] Gulhane Mil Med Acad, Inst Res & Dev, Ankara, Turkey
关键词
bleomycin; proanthocyanidin; pulmonary fibrosis; iNOS; hydroxyproline; inflammation; INDUCED LUNG FIBROSIS; RENAL ISCHEMIA/REPERFUSION INJURY; GRAPE SEED; OXIDATIVE STRESS; N-ACETYLCYSTEINE; ANTIOXIDANT ACTIVITY; NITRIC-OXIDE; KAPPA-B; BLEOMYCIN; MICE;
D O I
10.1007/s10753-012-9453-6
中图分类号
Q2 [细胞生物学];
学科分类号
071013 [干细胞生物学];
摘要
Pulmonary fibrosis (PF) is a progressive fatal disorder. Bleomycin (BLM) is a widely used chemotherapeutic agent causing PF. Numerous agents have been investigated to prevent the progression of PF so far, but there is still a need to find more efficacious agents. Proanthocyanidin (PA) is a strong antioxidant, the main ingredient of grape seed extract. Since PA is ready for use in practice, we aimed to compare the preventive effect of PA in comparison with taurine (Tau) in BLM-induced PF. Forty Wistar male albino rats were used in the study and were divided into four groups: group 1, control; group 2, BLM-induced PF group; group 3, BLM-induced PF and treated with PA group; and group 4, BLM-induced PF and treated with Tau group. Treatments were begun 10 days before and continued 21 days after BLM injection. PA and Tau effectively inhibited inflammation, edema, severity of fibrosis, fibrosis extension, inflammatory cell accumulation, iNOS staining, and hydroxyproline level as well (p < 0.05). Total histological scores of the PA group were similar to the control group; Tau was significantly higher than the control group but lower than the BLM group (p < 0.05). We believe that PA could be a new treatment choice for PF, but further studies need to be conducted to verify the findings of the current study.
引用
收藏
页码:1402 / 1410
页数:9
相关论文
共 52 条
[1]
Low molecular proanthocyanidin dietary biofactor Oligonol: Its modulation of oxidative stress, bioefficacy, neuroprotection, food application and chemoprevention potentials [J].
Aruoma, Okezie I. ;
Sun, Buxiang ;
Fujii, Hajime ;
Neergheen, Vidushi S. ;
Bahorun, Theeshan ;
Kang, Kyung-Sun ;
Sung, Mi-Kyung .
BIOFACTORS, 2006, 27 (1-4) :245-265
[2]
Bagchi D, 1997, RES COMMUN MOL PATH, V95, P179
[3]
Molecular mechanisms of cardioprotection by a novel grape seed proanthocyanidin extract [J].
Bagchi, DB ;
Sen, CK ;
Ray, SD ;
Das, DK ;
Bagchi, M ;
Preuss, HG ;
Vinson, JA .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2003, 523 :87-97
[4]
Subchronic 3-month oral toxicity study of grape seed and grape skin extracts [J].
Bentivegna, SS ;
Whitney, KM .
FOOD AND CHEMICAL TOXICOLOGY, 2002, 40 (12) :1731-1743
[5]
Blaisdell R J, 1995, J Biochem Toxicol, V10, P203, DOI 10.1002/jbt.2570100404
[6]
Interferon-γ1b for the treatment of idiopathic pulmonary fibrosis [J].
Bouros, Demosthenes ;
Antoniou, Katerina M. ;
Tzouvelekis, Argyris ;
Siafakas, Nikolaos M. .
EXPERT OPINION ON BIOLOGICAL THERAPY, 2006, 6 (10) :1051-1060
[7]
EFFECT OF DEOXYRIBONUCLEIC-ACID ON THE PRODUCTION OF REDUCED OXYGEN BY BLEOMYCIN AND IRON [J].
CASPARY, WJ ;
LANZO, DA ;
NIZIAK, C .
BIOCHEMISTRY, 1982, 21 (02) :334-338
[8]
Pharmacologic differentiation of inflammation and fibrosis in the rat bleomycin model [J].
Chaudhary, NI ;
Schnapp, A ;
Park, JE .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2006, 173 (07) :769-776
[9]
Short courses of low dose dexamethasone delay bleomycin-induced lung fibrosis in rats [J].
Chen, FP ;
Gong, LK ;
Zhang, L ;
Wang, H ;
Qi, XM ;
Wu, XF ;
Xiao, Y ;
Cai, Y ;
Liu, LL ;
Li, XH ;
Ren, J .
EUROPEAN JOURNAL OF PHARMACOLOGY, 2006, 536 (03) :287-295
[10]
Antioxidant activity of natural flavonoids is governed by number and location of their aromatic hydroxyl groups [J].
Chen, ZY ;
Chan, PT ;
Ho, KY ;
Fung, KP ;
Wang, J .
CHEMISTRY AND PHYSICS OF LIPIDS, 1996, 79 (02) :157-163